Difference in onset of warm ischemia and reperfusion injury between parenchymal and endothelial cells of the liver. Evaluation by purine nucleoside phosphorylase and hyaluronic acid

The Journal of Surgical Research
M NakagamiY Yamaoka

Abstract

The onset of warm ischemia and reperfusion injury in the liver was investigated in a canine model through changes in parenchymal markers [isozyme class V of lactate dehydrogenase (LDH) and alanine aminotransferase (ALT)], endothelial markers [purine nucleoside phosphorylase (PNP) and hyaluronic acid clearance], and the liver metabolism (ketone body ratio) in warm ischemia induced by inflow occlusion using Pringle's maneuver and subsequent reperfusion. In this in vivo model, a PNP assay system and a model were designed so as to exclude the influence of wide localization of PNP possibly originating in erythrocytes or the intestine, and to discriminate between PNP of endothelial cells and that of parenchymal cells in the liver. After 45 min of warm ischemia, reperfusion resulted in damage only to endothelial cells, as seen by significant increase in PNP alone (3.6 +/- 0.1 U/liter at the end of warm ischemia to 6.8 +/- 0.5 U/liter at 5 min after reperfusion, P < 0.01) and significant decrease in hyaluronic acid clearance compared to the 30-min warm ischemia group in which no increase in either marker for parenchymal and endothelial cells was noted. By contrast, after 60 min of warm ischemia, reperfusion resulted in damage to parenc...Continue Reading

Citations

Dec 31, 1998·Transplant International : Official Journal of the European Society for Organ Transplantation·J C García-ValdecasasJ Visa
Mar 19, 2003·Liver International : Official Journal of the International Association for the Study of the Liver·Bob H M HeijnenThomas M van Gulik
Mar 3, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C H CottartJ P Clot
May 5, 2001·Pharmacology & Therapeutics·A BzowskaD Shugar

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