Abstract
Osteopontin is a sialoprotein that is expressed in various cells. It plays a variety of important roles in cell adhesion, migration, signaling, calcification, and immunity. Its diverse functions indicate that the regulation of osteopontin may also vary extensively among tissues. Although osteopontin promoter has been studied in vitro, in vivo analyses may be more appropriate for elucidating osteopontin's functions. In an attempt to investigate osteopontin gene expression, we generated transgenic mice in which the bacterial beta-galactosidase reporter gene was conjugated downstream of osteopontin promoter. The osteopontin promoter was a mouse -910 bp upstream fragment, which we had previously found functional in 3T3 cells. Among 34 transgenic founders, 13 mice were transgenic, as determined with the polymerase chain reaction. Osteopontin and beta-galactosidase signals were evaluated with in situ hybridization. Among the 13 transgenic mice, 3 were beta-galactosidase-positive. In these transgenic mice, osteopontin signals were observed in bones and kidneys, whereas beta-galactosidase message was detected only in bones. This suggests that the -910 bp osteopontin promoter is active in bones but not in kidneys. These data imply that ...Continue Reading
References
Dec 1, 1991·Bone and Mineral·D N Kalu
Jul 1, 1989·The Journal of Experimental Medicine·R PatarcaH Cantor
Feb 1, 1988·The Journal of Cell Biology·S NomuraB L Hogan
Dec 15, 1985·The Biochemical Journal·A Franzén, D Heinegård
Oct 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K HirakawaS Nomura
Oct 1, 1994·The Biochemical Journal·N HijiyaS Yamamoto
Jun 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T IkedaS Yoshiki
May 5, 1997·FEBS Letters·M OkabeY Nishimune
Oct 10, 1997·Human Gene Therapy·D J WeissJ G Clark
Feb 7, 1998·Kidney International·W MinJ R Hoyer
Feb 7, 1998·Kidney International·J R AsplinJ R Hoyer
Aug 26, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M SatoT Ochi
Oct 30, 1998·Oncogene·M SatoS Nomura
Jun 3, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K TeraiS Nomura
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·H YoshitakeM Noda
Dec 29, 2000·Oncogene·D WangC L Gladson
Jan 6, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·H GuoP C Kuo
Mar 7, 2001·Kidney International·Y XieF Gejyo
Mar 29, 2001·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·W A VerstrepenM E De Broe
Apr 25, 2001·Kidney International·U PanzerR A Stahl
Apr 25, 2001·Kidney International·P E RayR J Johnson
May 1, 2001·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Y NagasawaM Hori
Apr 19, 2002·Journal of the American Society of Nephrology : JASN·Anja VerhulstMarc E De Broe
May 29, 2002·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Darren J KellyRichard E Gilbert
Citations
Feb 14, 2008·Endocrine Journal·Takafumi TaguchiKozo Hashimoto
Feb 3, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Yuji HigashibataShintaro Nomura
Jun 7, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Shinji FujiharaKeiji Moriyama