Differences between neonates and adults in carbohydrate sequences and reaction kinetics of plasmin and alpha(2)-antiplasmin

Thrombosis Research
M RiesP J Gaffney

Abstract

This study investigates reaction kinetics by slow-binding kinetics methods of both adult and fetal plasmin (Types 1 and 2) with adult and fetal alpha(2)-antiplasmin. In addition, carbohydrate sequences of Fetal and Adult Plasminogen Types 1 and 2, as well as fetal and adult alpha(2)-antiplasmin, were determined by mass spectrometric analysis. All curves of plasmin-alpha(2)-antiplasmin interaction followed the same pattern, indicating reversible slow-binding inhibition with an initial loose complex and a following tight complex. Differences between fetal and adult plasmin reactions with alpha(2)-antiplasmin were predominantly due to the initial loose complex. Values for K(i initial) in the reaction with adult alpha(2)-antiplasmin were 1.5 and 1.6 nM for Fetal Plasmin Types 1 and 2, respectively; compared to 0.3 and 0.7 nM for the corresponding adult types. Increasing concentrations of tranexamic acid resulted in a continuous increase of K(i initial) until a plateau was reached which was similar for all plasmin types. Almost identical values could be obtained when fetal alpha(2)-antiplasmin was used instead of adult alpha(2)-antiplasmin. Mass spectrometric analyses of the glycans present on plasminogen revealed a higher level of ...Continue Reading

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Citations

Mar 22, 2006·Acta Haematologica·Nagina ParmarAnthony K C Chan
Jan 30, 2010·Cellular and Molecular Life Sciences : CMLS·Simon S GerberJohann Schaller
Dec 8, 2010·Cellular and Molecular Life Sciences : CMLS·Johann Schaller, Simon S Gerber
May 20, 2006·Thrombosis Research·Manuela Albisetti
Nov 30, 2016·International Journal of Molecular Sciences·Peter Goettig
Dec 16, 2010·Paediatric Anaesthesia·Michael P Eaton, Ellen M Iannoli
Mar 20, 2008·Anesthesia and Analgesia·Michael P Eaton
Jan 11, 2021·Paediatric Anaesthesia·Philip WolstencroftBrian J Anderson
Jun 4, 2010·Anesthesia and Analgesia·Heather G YurkaUNKNOWN Congenital Heart Surgery Research Interest Group
Mar 26, 2010·Biologicals : Journal of the International Association of Biological Standardization·C Thelwell

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