PMID: 12770324May 29, 2003Paper

Differences between recombinant PTTH and crude brain extracts in cAMP-mediated ecdysteroid secretion from the prothoracic glands of the silkworm, Bombyx mori

Journal of Insect Physiology
Skarlatos G DedosH Kataoka

Abstract

The ability of recombinant prothoracicotropic hormone (rPTTH) or crude brain extract (cBRAIN) of Bombyx mori to stimulate ecdysteroid secretion from prothoracic glands (PGs) was investigated throughout the fifth instar and the first day of the pupal stage. Crude brain extracts could stimulate much higher ecdysteroid secretion than rPTTH during a 2h incubation. Recombinant PTTH did not increase the level of glandular cyclic AMP, except on days 4 and 5 of the fifth instar. Glandular cAMP levels were increased by cBRAIN from day 0 until day 5 of the fifth instar with the highest increase on day 3. On this day, rPTTH could not stimulate any increase of ecdysteroid secretion from the PGs during a 30min incubation. On the contrary, PGs incubated with cBRAIN for 30min showed increased secretory activity. Furthermore, on day 3 and in the absence of extracellular Ca(2+), rPTTH did not increase the glandular cAMP levels but cBRAIN did. Recombinant PTTH-stimulated ecdysteroid secretion from day 3 PGs was dependent on extracellular Ca(2+) in a dose-dependent manner. However, cBRAIN could stimulate ecdysteroid secretion even in the absence of extracellular Ca(2+). Taken together, the results of these experiments suggest the presence of a pr...Continue Reading

References

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Sep 1, 1988·Molecular and Cellular Endocrinology·V H MellerL I Gilbert
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Citations

Dec 16, 2004·Archives of Insect Biochemistry and Physiology·Shi-Hong Gu, Yien-Shing Chow
May 16, 2001·General and Comparative Endocrinology·S G DedosM Takamiya
Nov 6, 2003·Insect Biochemistry and Molecular Biology·Keiko Takaki, Sho Sakurai
May 29, 2003·Journal of Insect Physiology·S G. DedosH Fugo
May 19, 2009·Journal of Insect Physiology·Rita Pfister-Wilhelm, Beatrice Lanzrein

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