Differences between sites of binding to DNA and strand cleavage for complexes of bleomycin with iron or cobalt.

Journal of Molecular Recognition : JMR
M J McLeanM J Waring

Abstract

The sequence specificity of bleomycin A5 and of its light-activated cobalt complex were compared by examining the relative cleavage of each strand of two DNA fragments by either species. Significant differences between the two metallobleomycins were observed. The iron-bleomycin (Fe-BLM) complex cleaved the DNA molecules preferentially at dinucleotides GpT and GpC, whereas the light-activated cobalt-bleomycin complex (Co-BLM) showed a preference for cutting at the dinucleotide GpA in addition to cleavage at every GpT dinucleotide. Further, new sites of preferential cleavage were noted for Co-BLM in regions of the DNA where enhanced reaction with DNAaseI can be observed in the presence of the antibiotic. No differences in the cutting behaviour of the Fe-BLM were evident upon irradiation of the reaction mixture. A reduction in the relative efficiency of cutting at GpC sequences by Co-BLM is responsible for the previously observed diminution of double-strand breaks under conditions of photoactivated cleavage. The results are discussed in terms of the likely production of highly reactive, diffusible cutting elements in the light activated reaction which cause cleavage of the DNA in regions where the antibiotic is not bound.

References

Jul 11, 1978·Biochemistry·E A SausvilleS B Horwitz
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A D D'Andrea, W A Haseltine
Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M TakeshitaH Ohtsubo
May 1, 1987·The Biochemical Journal·K R FoxM J Waring
Dec 1, 1980·Journal of Medicinal Chemistry·E A RaoD H Petering
Dec 7, 1982·Biochemistry·C H Chang, C F Meares
Dec 17, 1984·European Journal of Biochemistry·K R Fox, M J Waring
Jun 1, 1984·Cell·H R Drew, A A Travers

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Citations

Mar 26, 2008·Proceedings of the National Academy of Sciences of the United States of America·Kristie D GoodwinMillie M Georgiadis
Feb 15, 1994·European Journal of Biochemistry·K P Nightingale, K R Fox
Nov 1, 1994·Bioconjugate Chemistry·S M Hecht
Dec 8, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Long H Chung, Vincent Murray
Jan 1, 1991·Critical Reviews in Biochemistry and Molecular Biology·E Palecek
Mar 10, 2009·Drug Delivery·T J MortonH Redl
Feb 1, 1990·Journal of Molecular Recognition : JMR·P E Nielsen
May 8, 2018·International Journal of Molecular Sciences·Vincent MurrayLong H Chung
Dec 26, 2001·Chemical Reviews·C A Claussen, E C Long
Jan 17, 2009·Journal of the American Chemical Society·Qian MaSidney M Hecht
Jul 1, 1992·Toxicology and Applied Pharmacology·D Beyersmann, A Hartwig
Nov 5, 2010·Journal of the American Chemical Society·Rachel A Giroux, Sidney M Hecht
Jan 29, 2000·Journal of Natural Products·S M Hecht
Aug 21, 2003·Journal of the American Chemical Society·Michael J RishelSidney M Hecht

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