Differences between Solution and Membrane Forms of Chitosan on the In Vitro Activity of Fibroblasts

Balkan Medical Journal
Bahar UsluAyhan Bilir

Abstract

Chitosan, a linear polysaccharide, has been recently used in biomedical applications. In vitro studies have demonstrated its effect on cellular growth and its stimulatory action on cellular layer formation. The present study aims to compare the proliferative effects of chitosan in two forms, membranous and solution forms, on Swiss 3T3 mouse embryonic fibroblasts. In vitro study. Three experimental groups were formed: cells were cultured in a normal medium without chitosan (Control Group); cells were cultured either in a medium containing 2.0% chitosan in membranous form (Membrane Group) or chitosan solution at a concentration of 2.0% (Solution Group). Two different methods were used in the experiments: cells cultured on the medium containing chitosan in solution or membranous forms (method 1); and chitosan solution or membranous forms were added into the medium containing previously cultured cells (method 2). Scanning electron microscopic investigations of the experimental groups revealed cells with well-defined cellular projections, intact cellular membranes and tight intercellular junctions. They were especially prominent in the membrane group of method 1 and in the membrane and solution groups of method 2. Mouse monoclonal a...Continue Reading

Citations

Nov 3, 2015·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·B UsluA Bilir
Mar 8, 2018·Journal of Physiology and Biochemistry·Tugba SoydasGonul Kanigur Sultuybek

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