Differences in adhesion and protrusion properties correlate with differences in migration speed under EGF stimulation.

BMC Biophysics
Yue HouIan C Schneider

Abstract

Cell migration plays an essential role in many biological processes, such as cancer metastasis, wound healing and immune response. Cell migration is mediated through protrusion and focal adhesion (FA) assembly, maturation and disassembly. Epidermal growth factor (EGF) is known to enhance migration rate in many cell types; however it is not known how FA maturation, FA dynamics and protrusion dynamics are regulated during EGF-induced migration. Here we use total internal reflection fluorescence (TIRF) microscopy and image analysis to quantify FA properties and protrusion dynamics under different doses of EGF stimulation. EGF was found to broaden the distribution of cell migration rates, generating more fast and slow cells. Furthermore, groups based on EGF stimulation condition or cell migration speed were marked by characteristic signatures. When data was binned based on EGF stimulation conditions, FA intensity and FA number per cell showed the largest difference among stimulation groups. FA intensity decreased with increasing EGF concentration and FA number per cell was highest under intermediate stimulation conditions. No difference in protrusion behavior was observed. However, when data was binned based on cell migration speed...Continue Reading

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Citations

Oct 4, 2013·Integrative Biology : Quantitative Biosciences From Nano to Macro·Laura Lara Rodriguez, Ian C Schneider
Mar 4, 2014·PloS One·John G LockStaffan Strömblad
Mar 31, 2015·The Journal of Surgical Research·Liliana PalenciaCiara Leydon
Sep 13, 2018·Integrative Biology : Quantitative Biosciences From Nano to Macro·Vivi AndasariMian Long
Sep 27, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Amanda S PelloweAnjelica L Gonzalez
Aug 11, 2017·Annual Review of Cell and Developmental Biology·Peter N DevreotesYuchuan Miao
Sep 16, 2014·Physical Biology·Yue HouIan C Schneider
Dec 15, 2019·Seminars in Cell & Developmental Biology·Xiaoguang LiPeter N Devreotes

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