Differences in aromatase expression and steroid hormone concentrations in the reproductive tissues of male domestic turkeys (Meleagris gallopavo) with white and yellow semen

British Poultry Science
L PardyakB Bilinska

Abstract

1. To show hormonal differences between male turkeys with yellow semen syndrome (YSS) and white, normal semen (WNS), the expression of aromatase, oestrogen receptor α (ERα), and oestrogen receptor β (ERβ) as well as testosterone and oestradiol concentrations in YSS and WNS testes, epididymis, and ductus deferens were examined. 2. To measure gene expression levels of aromatase and oestrogen receptors (ERs), three complementary techniques (real-time PCR, Western blot, and immunohistochemistry) were used, whereas steroid hormone levels were determined radio-immunologically. 3. Upregulation of aromatase and ERα mRNAs in YSS testes (P < 0.05; P < 0.01), epididymis (P < 0.001; P < 0.001), and ductus deferens (P < 0.05; P < 0.01) compared to those of WNS tissues was detected. Significant increases in the levels of aromatase and ERα proteins were detected in YSS testes (P < 0.001; P < 0.05), epididymis (P < 0.001; P < 0.001), and ductus deferens (P < 0.001; P < 0.05). The expression of ERβ mRNA and protein level was upregulated in the testes (P < 0.05; P < 0.01) and epididymis (P < 0.001; P < 0.01) but not in ductus deferens where it was downregulated (P < 0.01; P < 0.01). Increased intensity of immunoreactive proteins in YSS versus WN...Continue Reading

References

Oct 1, 1984·Poultry Science·R A Hess, R J Thurston
Oct 1, 1984·General and Comparative Endocrinology·G V CallardR Reinboth
Sep 1, 1982·Poultry Science·R J ThurstonH V Biellier
Mar 1, 1995·Cell and Tissue Research·W D SierraltaH H Thole
Dec 1, 1995·Biology of Reproduction·S KwonJ M Bahr
Apr 1, 1997·The Journal of Steroid Biochemistry and Molecular Biology·G ShettyR N Moudgal
Dec 11, 1997·Nature·R A HessD B Lubahn
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·K M RobertsonE R Simpson
Aug 12, 1999·International Journal of Andrology·S CarreauJ Levallet
Jun 16, 2001·Endocrine Reviews·L O'DonnellE R Simpson
Jun 19, 2001·Molecular and Cellular Endocrinology·R A HessJ Bahr
Jan 22, 2002·Reproduction, Fertility, and Development·R A HessK Carnes
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 2, 2002·Journal of Computational Chemistry·Andre Elvas Pereira da Silva, Geraldo Magela E Silva
Jul 11, 2002·The Journal of Clinical Endocrinology and Metabolism·Saveria AquilaSebastiano Andò
Jul 20, 2002·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·D R HolsbergerC D Rice
Feb 28, 2003·Molecular Human Reproduction·Sophie LambardSerge Carreau
May 16, 2003·Reproductive Biology and Endocrinology : RB&E·Serge CarreauSonia Bourguiba
Mar 9, 2004·Aquatic Toxicology·Martina Fenske, Helmut Segner
Feb 3, 2005·Asian Journal of Andrology·Vincenzo RochiraCesare Carani
Feb 11, 2005·Archives of Histology and Cytology·Yoshiro ToyamaKiyotaka Toshimori
Sep 29, 2005·Reproductive Biology and Endocrinology : RB&E·Benson T Akingbemi
Feb 21, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Wen-Hung TwanChing-Fong Chang
Jul 11, 2006·Frontiers in Neuroendocrinology·Paul M ForlanoAndrew H Bass
Dec 25, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Malgorzata Kotula-BalakBarbara Bilińska
Mar 29, 2008·Current Genomics·Maria MarinoPaolo Ascenzi
Apr 15, 2008·Trends in Endocrinology and Metabolism : TEM·R M Sharpe
Feb 4, 2010·Arquivos brasileiros de endocrinologia e metabologia·Maria Fatima Magalhães LazariCatarina Segreti Porto
Apr 3, 2010·BioTechniques·Jennifer BordeauxDavid Rimm
Apr 21, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Serge Carreau, Rex A Hess
Apr 21, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Serge CarreauIsabelle Galeraud-Denis
Sep 21, 2010·Reproductive Toxicology·Anna HejmejBarbara Bilińska
Feb 10, 2012·Spermatogenesis·Serge CarreauChristelle Delalande
Apr 5, 2014·General and Comparative Endocrinology·Malgorzata Kotula-BalakBarbara Bilinska

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
PCR
Assay

Software Mentioned

ImageJ
Image LabTM

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.