Differences in expression pattern and function between zebrafish hoxc13 orthologs: recruitment of Hoxc13b into an early embryonic role

Developmental Biology
Ryan ThummelAlan R Godwin

Abstract

Vertebrate Hox genes are generally believed to initiate expression at the primitive streak or early neural plate stages. The timing and spatial restrictions of the Hox expression patterns during these stages correlate well with their demonstrated role in axial patterning. Here we demonstrate that one zebrafish hoxc13 ortholog, hoxc13a, has an expression pattern in the developing tail bud that is consistent with the gene playing a role in axial patterning. However, the second hoxc13 ortholog, hoxc13b, is maternally expressed and is detectable in every cell of early cleavage embryos through gastrulae. In addition, both transcript and protein are detectable at these stages. At 19 h post fertilization (hpf), hoxc13b expression is up-regulated in the tail bud, becoming restricted to the tail bud by 24 hpf. Importantly, by 24 hpf, hoxc13b morphants show a specific developmental delay, which can be rescued by co-injecting synthetic capped hoxc13a or hoxc13b message. These data suggest some functional divergence due to altered expression patterns of the two hoxc13 orthologs after duplication. Further characterization of the hoxc13b morphant delay reveals that it is biphasic in nature, with the first phase of the delay occurring before ...Continue Reading

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Citations

Apr 13, 2013·The International Journal of Biochemistry & Cell Biology·Ricardo Lara-RamírezMichael Schubert
Apr 18, 2007·Development Genes and Evolution·Ryan ThummelAlan R Godwin
Dec 29, 2007·Developmental Neurobiology·Ryan ThummelDavid R Hyde
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Mar 6, 2019·Nature Ecology & Evolution·Kosmas HenchOscar Puebla

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