Differences in F9 and 5.51 cell elasticity determined by cell poking and atomic force microscopy

FEBS Letters
Wolfgang Heinrich GoldmannR M Ezzell

Abstract

We studied the elasticity of both a wild type (F9) mouse embryonic carcinoma and a vinculin-deficient (5.51) cell line, which was produced by chemical mutagenesis. Using cell poking, we measured the effects of loss of vinculin on the elastic properties of these cells. F9 cells were about 20% more resistant to indentation by the cell poker (a glass stylus) than were 5.51 cells. Using the atomic force microscope to map the elasticity of wild type and vinculin-deficient cells by 128 X 128 force scans, we observed a correlation of elasticity with cell poking elastometric measurements. These findings, as well as previous atomic force, rheologic, and magnetometric measurements [Goldmann and Ezzell, Exp. Cell Res. 226 (1996) 234-237; Ezzell et al., Exp. Cell Res. 231 (1997) 14-26], indicate that vinculin is an integral part of the cytoskeletal network.

References

Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·J L CollE D Adamson
Feb 1, 1996·Journal of Muscle Research and Cell Motility·W H GoldmannG Isenberg
Feb 25, 1997·Experimental Cell Research·R M EzzellD E Ingber

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Citations

Jan 25, 2002·Cell Motility and the Cytoskeleton·M NagayamaK Kawabata
Jan 12, 2002·Biochemical and Biophysical Research Communications·Wolfgang H Goldmann, Donald E Ingber
Apr 4, 2003·Life Sciences·José Luis Alonso, Wolfgang H Goldmann
Oct 11, 2003·Biotechnology Advances·Z ReichY Scolnik
Nov 7, 2000·Colloids and Surfaces. B, Biointerfaces·I Lee, R E Marchant
Nov 13, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Stefania PuttiniNicolas Mermod
Sep 25, 2007·Biophysical Journal·Claudia Tanja MierkeWolfgang Heinrich Goldmann
Sep 6, 2011·Current Opinion in Cell Biology·Deborah E LeckbandJohan de Rooij
Dec 17, 2009·Biophysical Journal·Gerold DiezWolfgang H Goldmann
Mar 11, 2017·Journal of Cardiovascular Translational Research·Ariane C C van SpreeuwelCarlijn V C Bouten
Jul 25, 1998·FEBS Letters·A Vinckier, G Semenza

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