Differences in gene expression of human xylosyltransferases and determination of acceptor specificities for various proteoglycans

Biochemical and Biophysical Research Communications
Christina RochChristian Götting

Abstract

The xylosyltransferase (XT) isoforms XT-I and XT-II initiate the posttranslational glycosaminoglycan (GAG) synthesis. Here, we determined the relative expression of both isoforms in 33 human cell lines. The majority of tested cell lines showed dominant XYLT2 gene expression, while only in 23132/87, JAR, NCI-H510A and THP-1 was the XT-I mRNA expression higher. Nearly equal expression levels were detected in six cell lines. Additionally, to shed light on putative differences in acceptor specificities the acceptor properties of potential acceptor sequences were determined. Peptides were expressed as glutathione-S-transferase fusion proteins containing putative or known GAG attachment sites of in vivo proteoglycans. Kinetic analysis showed that K(m) and V(max) values for XT-I mediated xylosylation were slightly higher than those for XT-II, and that XT-I showed a lesser stringency concerning the acceptor sequence. Mutagenesis of the bikunin peptide sequence in the G-S-G attachment site and flanking regions generated potential acceptor molecules. Here, mutations on the N-terminal side and the attachment site were found to be more susceptible to a loss of acceptor function than mutations in the C-terminus. Altogether the known consens...Continue Reading

References

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Citations

Jun 23, 2012·Nature Reviews. Molecular Cell Biology·Kelley W MoremenAlison V Nairn
Mar 29, 2011·Cold Spring Harbor Perspectives in Biology·Pamela Stanley
Feb 5, 2011·BMC Biochemistry·Yasuo YamakoshiJames P Simmer
Dec 15, 2012·Connective Tissue Research·Casey FrankenbergerTheodore R Oegema
Oct 10, 2013·Journal of Pharmaceutical Sciences·David SpencerChristopher Barton
Mar 4, 2014·American Journal of Human Genetics·Catherine BuiValérie Cormier-Daire
Oct 13, 2016·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Huijuan LiuFusheng Dong
Dec 7, 2014·BMC Genetics·Isabel FaustDoris Hendig
Sep 24, 2020·Glycoconjugate Journal·Beatrix FerenczMyron E Hinsdale
Aug 14, 2012·Glycoconjugate Journal·Benjamin MüllerChristian Götting
Feb 20, 2019·International Journal of Obesity : Journal of the International Association for the Study of Obesity·Pulavendran SivasamiMyron E Hinsdale
Oct 23, 2020·Nature Reviews. Molecular Cell Biology·Katrine T SchjoldagerHenrik Clausen
Jun 9, 2020·Biochimica Et Biophysica Acta. General Subjects·Arnaud BruneelFrançois Fenaille

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