Differences in Krox20-dependent regulation of Hoxa2 and Hoxb2 during hindbrain development

Developmental Biology
M K MaconochieR Krumlauf

Abstract

During hindbrain development, segmental regulation of the paralogous Hoxa2 and Hoxb2 genes in rhombomeres (r) 3 and 5 involves Krox20-dependent enhancers that have been conserved during the duplication of the vertebrate Hox clusters from a common ancestor. Examining these evolutionarily related control regions could provide important insight into the degree to which the basic Krox20-dependent mechanisms, cis-regulatory components, and their organization have been conserved. Toward this goal we have performed a detailed functional analysis of a mouse Hoxa2 enhancer capable of directing reporter expression in r3 and r5. The combined activities of five separate cis-regions, in addition to the conserved Krox20 binding sites, are involved in mediating enhancer function. A CTTT (BoxA) motif adjacent to the Krox20 binding sites is important for r3/r5 activity. The BoxA motif is similar to one (Box1) found in the Hoxb2 enhancer and indicates that the close proximity of these Box motifs to Krox20 sites is a common feature of Krox20 targets in vivo. Two other rhombomeric elements (RE1 and RE3) are essential for r3/r5 activity and share common TCT motifs, indicating that they interact with a similar cofactor(s). TCT motifs are also found ...Continue Reading

References

Apr 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R Krumlauf
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·S NonchevR Krumlauf
May 8, 1997·Nature·M ManzanaresR Krumlauf
Oct 17, 1998·The EMBO Journal·J SvarenJ Milbrandt
Jul 9, 1999·Developmental Biology·M ManzanaresR Krumlauf
Jun 17, 2000·Results and Problems in Cell Differentiation·P A TrainorR Krumlauf

❮ Previous
Next ❯

Citations

Aug 3, 2005·Cellular and Molecular Neurobiology·Z N Akin, A J Nazarali
Dec 24, 2008·Proceedings of the National Academy of Sciences of the United States of America·Stefan TümpelLeanne M Wiedemann
Nov 13, 2008·Human Molecular Genetics·Susan E SwanbergJanine M LaSalle
Nov 17, 2011·BMC Evolutionary Biology·Alfonso NataleAnnamaria Locascio
Mar 30, 2006·Proceedings of the National Academy of Sciences of the United States of America·Stefan TümpelRobb Krumlauf
Oct 3, 2002·Evolution & Development·William R Jackman, Charles B Kimmel
Feb 22, 2012·Progress in Neurobiology·Philippe MorquetteArlette Kolta
Oct 14, 2008·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Adam DavisEdmund J Stellwag
Aug 14, 2013·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Dorothea Schulte, Dale Frank
Dec 17, 2002·Developmental Biology·Tai On YauMai Har Sham
May 11, 2005·Developmental Biology·Yasunori MurakamiShigeru Kuratani
Dec 26, 2009·Differentiation; Research in Biological Diversity·Sara Tavella, Nicoletta Bobola
Mar 7, 2006·Developmental Biology·Cecilia B Moens, Licia Selleri
Aug 5, 2017·Wiley Interdisciplinary Reviews. Developmental Biology·Hugo J Parker, Robb Krumlauf
Dec 7, 2018·Cellular and Molecular Life Sciences : CMLS·Dale Frank, Dalit Sela-Donenfeld
Mar 15, 2019·Nature Communications·Hugo J ParkerRobb Krumlauf
Apr 13, 2013·Development·Galya KayamDalit Sela-Donenfeld
May 19, 2021·Development Genes and Evolution·Allison M Fuiten, William A Cresko

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.