PMID: 9434283Jan 22, 1998Paper

Differences in lipoprotein lipid concentration and composition modify the plasma distribution of cyclosporine

Pharmaceutical Research
K M WasanLane J Brunner

Abstract

The purpose of this study was to define the relationship between lipoprotein (LP) lipid concentration and composition and the distribution of cyclosporine (CSA) in human plasma. 3H-CSA LP distribution was determined in normolipidemic human plasma that had been separated into different LP and lipoprotein-deficient plasma (LPDP) fractions by either affinity chromatography coupled with ultracentrifugation, density gradient ultracentrifugation or fast protein liquid chromatography. 3H-CSA LP distribution (at a concentration of 1000 ng/ml) was also determined in patient plasma samples with defined dyslipidemias. Furthermore, 3H-CSA LP distribution was determined in patient plasma samples of varying LP lipid concentrations. Following incubation, the plasma samples were separated into their LP and LPDP fractions by sequential phosphotungistic acid precipitation in the dyslipidemia studies and by density gradient ultracentrifugation in the specific lipid profile studies and assayed for CSA by radioactivity. Total plasma and lipoprotein cholesterol (TC), triglyceride (TG) and protein (TP) concentrations in each sample were determined by enzymatic assays. When the LP distribution of CSA was determined using three different LP separation ...Continue Reading

Citations

Sep 16, 2006·Pharmaceutical Research·Mohamad Firas BassissiAnne Lespine
Feb 11, 2010·The Journal of Veterinary Medical Science·Hisashi MizutaniHisashi Hirose
Apr 1, 2010·European Journal of Clinical Pharmacology·Rogier R PressHenk-Jan Guchelaar
Dec 19, 2019·International Journal of Molecular Sciences·Jaideep ChaudharyIan R Corbin
Jul 20, 2002·Clinical Pharmacokinetics·Fatemeh Akhlaghi, Andrew K Trull
Mar 5, 2004·British Journal of Clinical Pharmacology·H ZahirA J McLachlan
Jul 16, 2005·Memórias do Instituto Oswaldo Cruz·Maria Jose MorillaEder Lilia Romero
Feb 19, 2000·Antimicrobial Agents and Chemotherapy·J R RoseD P Rossignol
Nov 3, 2005·Environmental Health Perspectives·Ricky T WoofterJohn S Ramsdell
Dec 18, 2001·Journal of Pharmaceutical Sciences·K D Peteherych, K M Wasan
Apr 21, 1998·Journal of Pharmaceutical Sciences·K M Wasan, S M Cassidy
Apr 28, 2004·Advanced Drug Delivery Reviews·Nancy S Chung, Kishor M Wasan
Sep 6, 2005·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Pavel Gershkovich, Amnon Hoffman
Nov 29, 2005·Metabolism: Clinical and Experimental·Gianluca RuiuMaurizio Cassader
Nov 26, 2010·Journal of the American Academy of Dermatology·Caitriona RyanAlan Menter

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