Differences in MAP kinase phosphorylation in response to mechanical strain in asthmatic fibroblasts.

Respiratory Research
F Le BellegoMara S Ludwig

Abstract

Mechanical strain alters protein expression. It results in phosphorylation of MAP kinases and up-regulation of extracellular matrix proteins. We investigated whether phosphorylation of MAP kinase family members was increased in response to mechanical strain in fibroblasts from asthmatic patients (AF) and normal controls (NF), and whether phosphorylation of these signaling molecules would be different in the two cell populations. Fibroblasts were obtained from mild, atopic asthmatics and non-atopic volunteers using endobronchial biopsy. Cells were grown on flexible, collagen I-coated membranes, and subjected to mechanical strain (Flexercell). MAP kinase phosphorylation was measured at baseline, and during one hour of strain. We also examined the effect of strain on proteoglycan production. At baseline, there was increased phosphorylation of ERK1/2 and p38, and decreased phosphorylation of JNK in AF vs NF. During strain in NF, p38 phosphorylation was increased. Conversely in AF, strain resulted in an increase in JNK phosphorylation, had no effect on phosphorylation of p38, and resulted in a decrease in ERK1/2 phosphorylation. There was a significant increase in versican protein production after 24 h strain in both AF and NF. JNK ...Continue Reading

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Citations

Aug 14, 2009·Journal of Biomolecular Screening·Victor Sanjit Nirmalanandhan, G Sitta Sittampalam
Nov 21, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Alice A TomeiMelody A Swartz

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Methods Mentioned

BETA
biopsy
biopsies
flow cytometry
protein assay

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