Differences in metabolite profile between blood plasma and serum

Analytical Biochemistry
Linsheng LiuZimei Wu

Abstract

In metabolomic research, blood plasma and serum have been considered to possess similar compositions and properties. Their perceived equivalence has resulted in researchers choosing arbitrarily between serum and plasma for analysis. Here, routine serum and plasma were prepared and their low-molecular-weight compounds were determined using gas chromatography/time-of-flight mass spectrometry. Principal components analysis was applied to process the acquired data, and marked differences in metabolite profiles were observed between serum and plasma. Of the 72 identified compounds, 36 (50%) discriminate serum from plasma, with 29 and 7 metabolites showing a significantly higher abundance (t test, P<0.05) in serum and plasma, respectively. Incubation of blood had distinct effects on the analyte peak areas, with the effects being more pronounced for plasma than for serum and more pronounced for a shorter incubation than for a longer incubation. These results highlight the importance in choosing serum or plasma as the analytical sample and in stipulating the incubation time. Because incubation affected the analyte peak areas less in serum than in plasma, we recommend serum as the sample of choice in metabolomic studies.

References

Aug 1, 1991·Circulation·L Wilhelmsen
Aug 28, 2004·Clinical Chemistry·Rodney R MilesWilliam L Roberts
Dec 15, 2005·Analytical Chemistry·Jiye AThomas Moritz
Feb 3, 2007·Journal of Proteome Research·Johan TryggTorbjörn Lundstedt
May 27, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ying ZhangLongsheng Sheng

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Citations

Jul 16, 2011·PloS One·Zhonghao YuRui Wang-Sattler
Jul 7, 2015·Biomedical Chromatography : BMC·Linsheng LiuGuangji Wang
Feb 4, 2016·Talanta·M A López-BascónM D Luque de Castro
Jan 26, 2016·Journal of Pharmaceutical and Biomedical Analysis·Ya-Xiao ChenDong-Zi Yang
Jul 31, 2012·Drug and Alcohol Dependence·Tian ZhengXuanxuan Zhu
Apr 14, 2016·Metabolomics : Official Journal of the Metabolomic Society·Desirée HaoAalim M Weljie
Aug 30, 2014·Proteomics·Frédérique CourantBruno Le Bizec
Mar 13, 2014·Biomedical Chromatography : BMC·Zhang LinYizeng Liang
Aug 13, 2013·Biomedical Chromatography : BMC·Saray Ly-VerdúRalf Zimmermann
Mar 5, 2015·Analytical and Bioanalytical Chemistry·Peiyuan YinGuowang Xu
Jun 3, 2016·Frontiers in Physiology·Catherine G VasilopoulouMaria I Klapa
Jun 22, 2016·Bioanalysis·Isabelle KohlerMartin Giera
Jul 5, 2016·Biological & Pharmaceutical Bulletin·Kosuke SaitoYoshiro Saito
Jan 26, 2017·Alzheimer's & Dementia : the Journal of the Alzheimer's Association·Madhav ThambisettyCristina Legido Quigley
May 26, 2017·Electrophoresis·Vinicius Veri HernandesDanuta Dudzik
Jan 12, 2017·Scientific Reports·Raluca Georgiana MaltesenReinhard Wimmer
May 13, 2017·Journal of Pharmaceutical Sciences·Keiko MaekawaYoshiro Saito
Nov 4, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Guangyue SuJiao Bai
Nov 6, 2017·Journal of Dairy Science·P L HarrelsonT T Ross
Mar 31, 2011·Electrophoresis·Haleem J IssaqTimothy D Veenstra
Mar 31, 2015·Electrophoresis·Akiyoshi HirayamaToru Takebayashi
Sep 4, 2018·Molecular Nutrition & Food Research·Marynka M UlaszewskaGuy Vergères
Oct 13, 2018·Critical Reviews in Clinical Laboratory Sciences·Gabriel Lima-OliveiraGian Cesare Guidi
Dec 7, 2018·Metabolites·Charmion Cruickshank-QuinnNichole Reisdorph
Dec 15, 2017·Critical Reviews in Clinical Laboratory Sciences·C GarA Lechner
Nov 7, 2015·Molecular and Cellular Pediatrics·Marianne S Muhlebach, Wei Sha
May 30, 2019·Biomarkers in Medicine·Shiva Kalantari, Mohsen Nafar
Aug 12, 2019·Biomolecular Concepts·Akul MunjalSadanand Fulzele
May 18, 2020·Endocrinology·Brooke ArmisteadHamid-Reza Kohan-Ghadr
Aug 23, 2019·Scientific Reports·A S ByrdG A Okoye

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