Differences in Morphometric Measures of the Uninjured Porcine Spinal Cord and Dural Sac Predict Histological and Behavioral Outcomes after Traumatic Spinal Cord Injury

Journal of Neurotrauma
Kyoung-Tae KimBrian K Kwon

Abstract

One of the challenges associated with conducting experiments in animal models of traumatic spinal cord injury (SCI) is inducing a consistent injury with minimal variability in the degree of tissue damage and resultant behavioral and biochemical outcomes. We evaluated how the variability in morphometry of the spinal cord and surrounding cerebrospinal fluid (CSF) contributes to the variability in behavioral and histological outcomes in our porcine model of SCI. Using intraoperative ultrasound imaging, spinal cord morphometry was assessed in seven Yucatan minipigs undergoing a weight-drop T10 contusion-compression injury. Bivariate and multi-variate analysis and modeling were used to identify native morphometrical determinants of interanimal variability in histological and behavioral outcomes. The measured biomechanical impact parameters did not correlate with the histological measures or hindlimb locomotor behavior (Porcine Thoracic Injury Behavior Scale). In contrast, clear associations were revealed between CSF layer morphometry and the amount of white matter and tissue sparing. Specifically, the dorsoventral diameter of the dural sac and ventral CSF space were strong predictors of behavioral and histological outcome and togeth...Continue Reading

References

Sep 15, 1994·Spine·T KameyamaA Takahashi
Jan 25, 2006·Journal of Neurotrauma·John C GenselJacqueline C Bresnahan
May 16, 2009·Journal of Biomechanics·Elizabeth C ClarkeLynne E Bilston
Jun 9, 2010·Journal of Biomechanical Engineering·Benjamin S ElkinBarclay Morrison
Nov 5, 2010·Journal of Neurotrauma·Cecilia PerssonRichard M Hall
Nov 23, 2010·Journal of Neurotrauma·Kelly A DunhamCandace L Floyd
Sep 8, 2011·Journal of Applied Biomechanics·Cecilia PerssonRichard M Hall
Mar 30, 2012·Frontiers in Psychology·Amanda KrahaRobin K Henson
Jan 16, 2013·Journal of Neurotrauma·Jae H T LeeBrian K Kwon
Jul 31, 2013·Journal of Biomechanical Engineering·Claire F JonesPeter A Cripton
Jan 9, 2015·Journal of Neurotrauma·Femke StreijgerBrian K Kwon
Jan 21, 2016·Journal of Neurotrauma·Ernesto A SalegioMichael S Beattie
Jan 10, 2017·Frontiers in Neurology·Arvid FrostellMikael Svensson
Feb 19, 2017·Neuroscience Letters·Rustem Robertovich IslamovIgor Aleksandrovich Lavrov

❮ Previous
Next ❯

Methods Mentioned

BETA
PCA

Software Mentioned

Image J
Zen Imaging
Labview
XLSTAT

Related Concepts

Related Feeds

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.