Differences in nuclear thyroid hormone receptors among species

General and Comparative Endocrinology
K IchikawaT Yamada

Abstract

Hepatic nuclear thyroid hormone receptors from rat, dog, chicken, and rainbow trout were compared. Receptor affinities for 3,5,3'-triiodo-L-thyronine (T3) were similar in preparations from rat, dog, and chicken, using isolated nuclei and nuclear extracts. Rainbow trout nuclear receptor showed a lower affinity for T3. Almost half of the receptors were released into the medium with rat and chicken nuclei, and 79.7 +/- 1.1% of the receptors were released with rainbow trout nuclei, when isolated nuclei were incubated with T3 at 22 degrees for 2 hr. The affinity constant of rat liver receptor for calf thymus DNA-cellulose at 0.17 M KCl, pH 7.4, was 3.98 +/- 1.47 x 10(5) M-1, when determined using DNA-cellulose columns. The number of salt bridges involved in DNA binding of the rat receptor was 5.73 +/- 0.38. When receptor-DNA interactions were compared among species, significant differences were found, but the receptors from dog and rainbow trout liver were similar. Sephacryl S-200 column chromatography showed that chicken receptor had a Stokes radius significantly smaller than that of rat receptor. Partial proteolysis of T3-receptor complex using trypsin alpha-chymotrypsin, elastase, and papain produced distinct T3-binding fragments...Continue Reading

References

Apr 1, 1979·The Journal of Clinical Endocrinology and Metabolism·L D SchusterJ H Oppenheimer
May 1, 1975·Endocrinology·J Torresani, L J DeGroot
Nov 4, 1976·Journal of Molecular Biology·M T RecordP De Haseth
Dec 18, 1986·Nature·C WeinbergerR M Evans
May 1, 1987·Molecular and Cellular Endocrinology·K Ichikawa, L J DeGroot
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·K Ichikawa, L J DeGroot
Dec 1, 1980·General and Comparative Endocrinology·G J van der Kraak, J G Eales

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Citations

Dec 12, 2001·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·D M PowerG E Sweeney
Jan 1, 1991·Life Sciences·K Ichikawa, K Hashizume
Dec 1, 1991·General and Comparative Endocrinology·D J Donoghue, C G Scanes

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