Differences in radiosensitivity between three HER2 overexpressing cell lines

European Journal of Nuclear Medicine and Molecular Imaging
Ann-Charlott SteffenJ Carlsson

Abstract

HER2 is a potential target for radionuclide therapy, especially when HER2 overexpressing breast cancer cells are resistant to Herceptin(R) treatment. Therefore, it is of interest to analyse whether HER2 overexpressing tumour cells have different inherent radiosensitivity. The radiosensitivity of three often used HER2 overexpressing cell lines, SKOV-3, SKBR-3 and BT-474, was analysed. The cells were exposed to conventional photon irradiation, low linear energy transfer (LET), to characterise their inherent radiosensitivity. The analysis was made with clonogenic survival and growth extrapolation assays. The cells were also exposed to alpha particles, high LET, from (211)At decays using the HER2-binding affibody molecule (211)At-(Z(HER2:4))(2) as targeting agent. Assays for studies of internalisation of the affibody molecule were applied. SKOV-3 cells were most radioresistant, SKBR-3 cells were intermediate and BT-474 cells were most sensitive as measured with the clonogenic and growth extrapolation assays after photon irradiation. The HER2 dependent cellular uptake of (211)At was qualitatively similar for all three cell lines. However, the sensitivity to the alpha particles from (211)At differed; SKOV-3 was most resistant, SKBR-3...Continue Reading

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Citations

Aug 8, 2009·European Journal of Nuclear Medicine and Molecular Imaging·Amelie FondellLars Gedda
Jan 5, 2011·International Journal of Radiation Oncology, Biology, Physics·Helen HeyerdahlJostein Dahle
May 16, 2009·Molecular Biology Reports·Pei HeGuang-Xiu Lu
Aug 1, 2015·World Journal of Gastroenterology : WJG·Wen-Juan YangMei Yuan
Jun 4, 2021·Physics in Medicine and Biology·Teresa L PalmerOlaug Hjelstuen
Mar 11, 2010·Journal of Experimental & Clinical Cancer Research : CR·Anli ZhangQiang Wu

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