Differences in replication kinetics and cell tropism between neurovirulent and non-neurovirulent EHV1 strains during the acute phase of infection in horses

Veterinary Microbiology
Annick GryspeerdtH J Nauwynck

Abstract

Equine herpesvirus 1 (EHV1) replicates in the respiratory tract of horses, after which infected leukocytes transport virus throughout the body, resulting in abortion or nervous system disorders. Two EHV1 strains circulate in the field: neurovirulent and non-neurovirulent. To investigate differences in replication in the upper respiratory tract (URT), an experimental inoculation study in ponies was performed with both strains. Two groups of six ponies, were inoculated intranasally with 10(6.5) TCID(50) of either strain. Clinical signs, nasal shedding and viremia were evaluated. At early time points post-inoculation (pi), one pony of each group was euthanized. Tissues were collected for titration and immunostainings. Number and size of EHV1-induced plaques were calculated, and individual EHV1-infected cells were quantified and characterized. Inoculation with either strain resulted in nasal shedding and replication in several tissues of the URT. Both strains replicated in a plaquewise manner in epithelium of the nasal mucosa, but replication in epithelium of the nasopharynx was largely limited to non-neurovirulent EHV1. Plaques were never able to cross the basement membrane, but individual infected cells were noticed in the connec...Continue Reading

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Citations

Dec 3, 2014·Comparative Immunology, Microbiology and Infectious Diseases·Hossein Bannazadeh Baghi, Hans J Nauwynck
Mar 19, 2013·Veterinary Research·Wee Ming YeoTracy Stokol
Feb 2, 2012·The Journal of Infectious Diseases·Susan L StramerUNKNOWN NHLBI Retrovirus Epidemiology Donor Study-II (REDS-II)
Sep 29, 2015·New Zealand Veterinary Journal·Amj McFaddenI G Mayhew
Jun 22, 2011·Journal of Comparative Pathology·C M C MoriP C Maiorka
Nov 26, 2010·Veterinary Microbiology·S WilstermanL S Goehring
Jan 16, 2016·The Veterinary Record·Magda Dunowska
Jan 18, 2019·Journal of Virology·Katrien C K PoelaertHans J Nauwynck
Sep 7, 2019·The Journal of General Virology·Katrien C K PoelaertHans J Nauwynck
Jan 31, 2020·Journal of Virology·Jolien Van CleemputHans J Nauwynck
Apr 30, 2017·Archives of Virology·Karol StasiakJerzy Rola
Apr 17, 2018·Equine Veterinary Journal·M E WilsonG Soboll Hussey
Sep 28, 2018·Frontiers in Cellular and Infection Microbiology·Katrien C K PoelaertHans J Nauwynck
Apr 27, 2019·Frontiers in Microbiology·Katrien C K PoelaertHans J Nauwynck
Dec 19, 2019·Frontiers in Microbiology·Fatai S OladunniThomas M Chambers
May 27, 2015·Transboundary and Emerging Diseases·H NegussieH J Nauwynck
Mar 23, 2021·Frontiers in Microbiology·Kathlyn LavalHans J Nauwynck

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