Differences in sexual development in inbred and outbred zebrafish (Danio rerio) and implications for chemical testing

Aquatic Toxicology
A Ross BrownCharles R Tyler

Abstract

Outbred laboratory animal strains used in ecotoxicology are intended to represent wild populations. However, breeding history may vary considerably between strains, driving differences in genetic variation and phenotypes used for assessing effects of chemical exposure. We compared a range of phenotypic endpoints in zebrafish from four different "breeding treatments" comprising a Wild Indian Karyotype (WIK) zebrafish strain and a WIK/Wild strain with three levels of inbreeding (F(IT)=n, n+0.25, n+0.375) in a new Fish Sexual Development Test (FSDT). There were no differences between treatments in terms of egg viability, hatch success or fry survival. However, compared with WIKs, WIK/Wild hybrids were significantly larger in size, with more advanced gonadal (germ cell) development at the end of the test (63 days post fertilisation). Increasing the levels of inbreeding in the related WIK/Wild lines did not affect body size, but there was a significant male-bias (72%) in the most inbred line (F(IT)=n+0.375). Conversely, in the reference WIK strain there was a significant female-bias in the population (80% females). Overall, our results support the use of outbred zebrafish strains in the FSDT, where one of the core endpoints is sex r...Continue Reading

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Citations

Jun 13, 2014·Environmental Toxicology and Chemistry·Olivier SimonSandrine Frelon
Jun 20, 2015·Journal of Toxicology and Environmental Health. Part a·Daniela LimaMiguel M Santos
Jun 1, 2018·Molecular Reproduction and Development·Thomas A Delomas, Konrad Dabrowski
Oct 16, 2018·Journal of Fish Biology·Thomas A Delomas, Konrad Dabrowski
Dec 7, 2019·Molecular Biology and Evolution·Jaanus SuurväliThomas Wiehe
Sep 23, 2014·Genetics·Catherine A WilsonJohn H Postlethwait
Feb 12, 2014·Environmental Science & Technology·Catherine A HarrisJohn P Sumpter

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