Differences in Sirtuin Regulation in Response to Calorie Restriction in Cryptococcus neoformans

Journal of Fungi
Tejas BouklasBettina C Fries

Abstract

Cryptococcus neoformans successfully replicates in low glucose in infected patients. In the serotype A strain, H99, growth in this condition prolongs lifespan regulated by SIR2, and can be modulated with SIR2-specific drugs. Previous studies show that lifespan modulation of a cryptococcal population affects its sensitivity to antifungals, and survival in an infection model. Sirtuins and their role in longevity are conserved among fungi; however, the effect of glucose starvation is not confirmed even in Saccharomyces cerevisiae. Lifespan analysis of C. neoformans strains in low glucose showed that 37.5% exhibited pro-longevity, and lifespan of a serotype D strain, RC2, was shortened. Transcriptome comparison of H99 and RC2 under calorie restriction demonstrated differences, confirmed by real-time PCR showing thatSIR2,TOR1,SCH9, andPKA1expression correlated with lifespan response to calorie restriction. As expected, RC2-sir2Δ cells exhibited a shortened lifespan, which was reconstituted. However, shortened lifespan from calorie restriction was independent ofSIR2. In contrast to H99 but consistent with alteredSIR2regulation,SIR2-specific drugs did not affect outcome of RC2 infection. These data suggest that SIR2 regulation and res...Continue Reading

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Citations

Dec 31, 2020·Journal of Fungi·Somanon BhattacharyaBettina C Fries
Jul 3, 2021·Journal of Fungi·Somanon BhattacharyaBettina C Fries

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Datasets Mentioned

BETA
GSE74298

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BETA
PCR

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Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

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