Differences in some properties of newborn and adult brain glutamate decarboxylase.

Journal of Neurobiology
R Tapia, G Meza-Ruíz

Abstract

Some properties of glutamate decarboxylase (EC 4.1.1.15) activity in brain of newborn and adult mouse were studied comparatively. It was found that glutamate decarboxylase of the newborn brain was strongly inactivated by homogenization in hypotonic medium, centrifugation of isotonic sucrose homogenates, preincubation at 37 degrees C or the addition of Triton-X-100, whereas the adult brain enzyme was practically unaffected by any of these conditions. It was also found that the newborn glutamate decarboxylase was less activated by pyridoxal 5'-phosphate and less inhibited by pyridoxal 5'-phosphate oxime-O-acetic acid, than the adult enzyme. These differences do not exist for brain dihydroxyphenylalanine decarboxylase (EC 4.1.1.26) and are not due to the release of inhibitors from the newborn brain. On the basis of the results obtained it is postulated that two forms of glutamate decarboxylase exist in brain: a newborn form, which is unstable and has high affinity for pyridoxal 5'-phosphate, and an adult form, which is much more stable and has low affinity for pyridoxal 5'-phosphate. The possible implications of these findings in the establishment of the gamma-aminobutyric acid dependent synaptic inhibitory mechanisms during devel...Continue Reading

References

Nov 1, 1970·Journal of Neurochemistry·K L Sims, F N Pitts
Dec 1, 1951·Proceedings of the Society for Experimental Biology and Medicine·E ROBERTSS FRANKEL

❮ Previous
Next ❯

Citations

Sep 1, 1987·Cellular and Molecular Neurobiology·D L Martin
Feb 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·L A DennerJ Y Wu
Sep 1, 1976·Journal of Neurochemistry·J Y WuA Schousboe
Jan 1, 1984·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·R M GrossfeldC F Baxter
May 20, 2004·Journal of Inorganic Biochemistry·Lidia Struzyńska, Grzegorz Sulkowski

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biosynthetic Transformations

Biosyntheic transformtions are multi-step, enzyme-catalyzed processes where substrates are converted into more complex products in living organisms. Simple compounds are modified, converted into other compounds, or joined together to form macromolecules. Discover the latest research on biosynthetic transformations here.