Differences in the ability of spermatozoa from individual boar ejaculates to withstand different semen-processing techniques

Animal Reproduction Science
I ParrillaJ Roca

Abstract

The present study aimed to evaluate the ability of spermatozoa from individual boar ejaculates to withstand different semen-processing techniques. Eighteen sperm-rich ejaculate samples from six boars (three per boar) were diluted in Beltsville Thawing Solution and split into three aliquots. The aliquots were (1) further diluted to 3×10(7) sperm/mL and stored as a liquid at 17°C for 72 h, (2) frozen-thawed (FT) at 1×10(9) sperm/mL using standard 0.5-mL straw protocols, or (3) sex-sorted with subsequent liquid storage (at 17°C for 6 h) or FT (2×10(7) sperm/mL using a standard 0.25-mL straw protocol). The sperm quality was evaluated based on total sperm motility (the CASA system), viability (plasma membrane integrity assessed using flow cytometry and the LIVE/DEAD Sperm Viability Kit), lipid peroxidation (assessed via indirect measurement of the generation of malondialdehyde (MDA) using the BIOXYTECH MDA-586 Assay Kit) and DNA fragmentation (sperm chromatin dispersion assessed using the Sperm-Sus-Halomax(®) test). Data were normalized to the values assessed for the fresh (for liquid-stored and FT samples) or the sorted semen samples (for liquid stored and the FT sorted spermatozoa). All of the four sperm-processing techniques affe...Continue Reading

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Citations

Nov 30, 2013·Reproductive Biology·Diego BucciMarcella Spinaci
Dec 4, 2012·International Journal of Molecular Sciences·Clara González-MarínRosa Roy
Jun 17, 2014·Theriogenology·Diego V AlkminJordi Roca
Oct 15, 2013·Reproduction in Domestic Animals = Zuchthygiene·C RodenasI Parrilla
Jun 28, 2015·Theriogenology·M SpinaciD Bucci
Jul 16, 2015·Reproduction in Domestic Animals = Zuchthygiene·J RocaA Bolarin
May 1, 2015·Asian-Australasian Journal of Animal Sciences·Peter F Surai, Vladimir I Fisinin
Jun 13, 2013·Theriogenology·Rocío Fernández-GagoFelipe Martínez-Pastor
Apr 26, 2016·Journal of Proteomics·Cristina Perez-PatiñoJordi Roca
Feb 13, 2015·Biology of Reproduction·Sule DoganErdoğan Memili
Feb 9, 2012·Archives of Sexual Behavior·Kenneth J Zucker
Aug 29, 2014·Reproduction, Fertility, and Development·Diego V AlkminJordi Roca
Jul 9, 2020·Frontiers in Veterinary Science·Arumugam KumaresanJane M Morrell
Sep 10, 2020·International Journal of Molecular Sciences·Lisa HöfnerDagmar Waberski
Jan 12, 2021·Journal of Animal Science and Biotechnology·Helen JäkelDagmar Waberski
Nov 15, 2020·Research in Veterinary Science·Isabel BarrancoXiomara Lucas
Apr 4, 2021·Animals : an Open Access Journal From MDPI·Estíbaliz LacalleFelipe Martínez-Pastor
Jun 15, 2021·Frontiers in Cell and Developmental Biology·Yentel Mateo-OteroIsabel Barranco
Jul 3, 2021·Animals : an Open Access Journal From MDPI·José Néstor CaamañoCarlos Olegario Hidalgo

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