Differences in the length of the carboxyl terminus mediate functional properties of neurokinin-1 receptor.

Proceedings of the National Academy of Sciences of the United States of America
Jian-Ping LaiSteven D Douglas

Abstract

The neurokinin-1 receptor (NK1R) has two naturally occurring forms that differ in the length of the carboxyl terminus: a full-length receptor consisting of 407 aa and a truncated receptor consisting of 311 aa. We examined whether there are differential signaling properties attributable to the carboxyl terminus of this receptor by using stably transfected human embryonic kidney (HEK293) cell lines that express either full-length or truncated NK1R. Substance P (SP) specifically triggered intracellular calcium increase in HEK293 cells expressing full-length NK1R but had no effect in the cells expressing the truncated NK1R. In addition, in cells expressing full-length NK1R, SP activated NF-kappaB and IL-8 mRNA expression, but in cells expressing the truncated NK1R, SP did not activate NF-kappaB, and it decreased IL-8 mRNA expression. In cells expressing full-length NK1R, SP stimulated phosphorylation of PKCdelta but inhibited phosphorylation of PKCdelta in cells expressing truncated NK1R. There are also differences in the timing of SP-induced ERK activation in cells expressing the two different forms of the receptor. Full-length NK1R activation of ERK was rapid (peak within 1-2 min), whereas truncated NK1R-mediated activation was s...Continue Reading

References

Oct 31, 1991·Biochemical and Biophysical Research Communications·B HopkinsA Graham
Sep 30, 1991·Biochemical and Biophysical Research Communications·Y TakedaJ E Krause
Apr 1, 1998·Journal of Neuroimmunology·C Palma, S Manzini
Sep 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A DeFeaN W Bunnett
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 26, 2003·Brain Research. Molecular Brain Research·Sarah J BakerIan L Gibbins
May 20, 2003·The European Journal of Neuroscience·Laura CaberlottoRenzo Carletti
May 22, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kristen PageSvetlana Iasvoyskaia
Sep 6, 2003·American Journal of Physiology. Cell Physiology·Fabien SchmidlinNigel W Bunnett
Nov 11, 2003·Clinical and Diagnostic Laboratory Immunology·Jian-Ping LaiWen-Zhe Ho
Jun 2, 2004·Biochemistry. Biokhimii︠a︡·O V Smirnova, R L Bogorad
Sep 18, 2004·Cell Death and Differentiation·F Chen
Apr 9, 2005·Clinical and Diagnostic Laboratory Immunology·Jian-Ping LaiWen-Zhe Ho
May 27, 2005·The Journal of Pharmacology and Experimental Therapeutics·Hon-Wai KoonCharalabos Pothoulakis
Jul 14, 2005·Cancer Chemotherapy and Pharmacology·Keisuke YamaguchiMadan M Kwatra
Mar 22, 2006·Proceedings of the National Academy of Sciences of the United States of America·Iordanes KaragiannidesCharalabos Pothoulakis
May 6, 2006·Proceedings of the National Academy of Sciences of the United States of America·J-P LaiS D Douglas
Oct 17, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ronald WilliamsGary W Hoyle
Jun 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Morris F TanskySusan E Leeman
Oct 10, 2007·Immunology·Marianna KulkaRobert P Schleimer

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Citations

Jul 24, 2012·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Xiaoyun FangMin Yu
Oct 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Earl GillespieJames M Becker
Jun 18, 2009·The Journal of Biological Chemistry·Graeme S CottrellNigel W Bunnett
Oct 14, 2010·Journal of Leukocyte Biology·Linda Monaco-ShawverJordan S Orange
Dec 29, 2010·International Journal of Inflammation·Akhil HegdeMadhav Bhatia
Jul 25, 2009·Cellular and Molecular Life Sciences : CMLS·Danijela Markovic, R A John Challiss
May 12, 2009·Trends in Immunology·Florin TulucSteven D Douglas
Dec 27, 2015·Neuropeptides·Eliska MistrovaMagdalena Chottova Dvorakova
Nov 26, 2010·Annals of the New York Academy of Sciences·Steven D Douglas, Susan E Leeman
Oct 1, 2015·BioMed Research International·Susana Garcia-Recio, Pedro Gascón
Aug 8, 2015·Mayo Clinic Proceedings·Folabomi A OladosuAndrea G Nackley
Jun 18, 2016·Cellular and Molecular Life Sciences : CMLS·Alireza MashaghiReza Dana
Oct 12, 2016·Journal of Neuroinflammation·Frances CorriganRobert Vink
Aug 23, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Susmit Suvas
Jan 19, 2018·Journal of Leukocyte Biology·Sergei SpitsinSteven D Douglas
Sep 28, 2018·Reviews in the Neurosciences·Prasanth M EapenMadhavan Nampoothiri
Apr 3, 2019·International Journal of Molecular Sciences·Chu-Ting ChangChih-Cheng Chen
Jan 3, 2014·Physiological Reviews·Martin S SteinhoffNigel W Bunnett
Sep 7, 2019·Proceedings of the National Academy of Sciences of the United States of America·Chentao GeCaiyun Fu
Nov 27, 2016·Oncotarget·Anton G HenssenJohannes H Schulte
Jun 17, 2018·Scientific Reports·Tanja NiedermairSusanne Grässel
May 31, 2020·Nature Communications·Julien WilsHervé Lefebvre
Jan 20, 2017·Frontiers in Cellular Neuroscience·M Brittany JohnsonIan Marriott
Jan 30, 2020·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Muhammad N A SahidKazutaka Maeyama
Mar 1, 2021·Journal of Neuroimmunology·Vasiliki PappaSteven D Douglas
Apr 25, 2021·Scientific Reports·Frances CorriganRobert Vink
Apr 15, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Zheng CaoNaiming Zhou

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