Differences in the metabolite composition and mechanical properties of extracellular vesicles secreted by hepatic cellular models

Journal of Extracellular Vesicles
Felix RoyoJuan M Falcón-Pérez

Abstract

Liver constitutes the major metabolic factory in the organism and is involved in the synthesis, secretion and clearance of many blood-circulating molecules. Previously, we have characterised the protein and RNA cargo of extracellular vesicles (EVs) secreted by two hepatic cellular models, a mouse hepatocyte progenitor cell line (MLP29) and primary rat hepatocytes (RHs). Here, we report the metabolome profile of these vesicles by performing a targeted UHPLC-MS metabolomics analysis of these two cellular models and their respective secreted EVs. Visual inspection of the data through principal component analysis allows clear separation of the metabolic profile of cells and EVs, and also of both cellular models. Correlation matrix supported that lipid composition of EVs is mainly determined by parent cell composition. EVs derived from MLP29 and RHs showed a negative correlation in their percentage composition of ceramides, glycerophospholipids, sphingomyelins and triglycerides. Metabolites enriched in EVs were also different depending on the cellular model. EVs secreted by MLP29 were enriched in different species of sphingomyelins and ceramides underrepresented in EVs secreted by RHs. Remarkably, triglycerides constitute an importa...Continue Reading

References

Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·B LindbladG Steen
Mar 1, 1996·The Journal of Experimental Medicine·G RaposoH J Geuze
Mar 3, 1986·Physical Review Letters·G BinnigC Gerber
Feb 14, 2006·Biophysical Journal·Albert J JinRalph Nossal
Apr 12, 2006·Proceedings of the National Academy of Sciences of the United States of America·J P MichelC F Schmidt
May 17, 2007·The Review of Scientific Instruments·I HorcasA M Baro
Jan 25, 2008·Nature Reviews. Molecular Cell Biology·Gerrit van MeerGerald W Feigenson
Mar 1, 2008·Science·Katarina TrajkovicMikael Simons
Mar 13, 2009·Cell Calcium·Trevor J Shuttleworth
Mar 31, 2009·Expert Opinion on Drug Delivery·Ellas SpyratouCostas Demetzos
Apr 16, 2009·Journal of Proteome Research·Javier Conde-VancellsJuan M Falcon-Perez
Oct 27, 2010·Proceedings of the National Academy of Sciences of the United States of America·Christopher W HarlandRaghuveer Parthasarathy
Jan 7, 2011·Cancer Biology & Therapy·Lindsay K RylandMark Kester
Oct 25, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Shivani SharmaJames K Gimzewski
Feb 24, 2012·Ultramicroscopy·A Ortega-EstebanJ Gómez-Herrero
Jun 16, 2012·Current Diabetes Reviews·Rob N M Weijers
Oct 3, 2012·Cold Spring Harbor Perspectives in Biology·Sima Lev
Nov 10, 2012·Metabolomics : Official Journal of the Metabolomic Society·Esperanza GonzalezJuan M Falcon-Perez
Jul 23, 2013·PloS One·Felix RoyoJuan M Falcon-Perez
Jan 1, 2012·Journal of Extracellular Vesicles·Felix Royo, Juan M Falcon-Perez
Sep 21, 2013·Biochimica Et Biophysica Acta·Alicia LlorenteKirsten Sandvig
Oct 22, 2013·Biochimica Et Biophysica Acta·Michel RecordSandrine Silvente-Poirot
Jan 10, 2014·Journal of the Royal Society, Interface·Shivani SharmaJames K Gimzewski
Sep 17, 2014·Nature Communications·Edward C HutchinsonErvin Fodor
Feb 11, 2015·Journal of Proteomics·Ibon Martínez-ArranzJosé M Mato
Apr 25, 2015·Methods : a Companion to Methods in Enzymology·Todd A LydicGavin E Reid
Mar 10, 2016·Developmental Cell·Aaron M HosiosMatthew G Vander Heiden
May 2, 2016·Chemistry and Physics of Lipids·Aritz B García-ArribasFelix M Goñi
Jul 21, 2016·Cellular and Molecular Life Sciences : CMLS·Nina Pettersen HessvikAlicia Llorente
Nov 5, 2016·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Felix RoyoJuan M Falcon-Perez
Nov 20, 2016·Journal of Extracellular Vesicles·Reka A HarasztiAnastasia Khvorova
Mar 28, 2017·Progress in Lipid Research·Tore SkotlandAlicia Llorente
Apr 15, 2017·Biochemical Society Transactions·Francisco Moreno-MadridPedro J de Pablo

❮ Previous
Next ❯

Citations

Apr 2, 2020·Cells·Alejandra HernándezMarco Arrese
Aug 14, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Cheng-Jie DengYun Zhang
Aug 6, 2020·International Journal of Molecular Sciences·Keigo NakamuraKazuhiko Imakawa
Sep 23, 2020·Pharmacology & Therapeutics·Maria Azparren-AnguloJuan M Falcon-Perez
Feb 23, 2020·Biochimica Et Biophysica Acta. General Subjects·Cristina ZivkoPaola Luciani
Nov 11, 2019·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·M Helena VasconcelosAija Linē
Jan 29, 2020·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·David García-SeisdedosRebeca Busto
May 1, 2021·International Journal of Molecular Sciences·Maria NotarnicolaCaterina Messa
May 27, 2021·Journal of Extracellular Vesicles·Uta ErdbrüggerElena S Martens-Uzunova
Jun 18, 2021·Nature Protocols·Guillermo Bordanaba-FloritJuan M Falcón-Pérez
Aug 7, 2021·Advanced Drug Delivery Reviews·Peiwen FuZhimin Tao

❮ Previous
Next ❯

Methods Mentioned

BETA
AFM
atomic force microscopy
atomic
electron microscopy
PCA
transmission electron microscopy

Software Mentioned

WSxM
corrplot
Factominer
Nanotec
R
TargetLynx
MassLynx

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved