PMID: 11898346Mar 20, 2002Paper

Differences in the microflora of scarified and unscarified seeds of Karwinskia humboldtiana (Rhamnaceae)

Folia Microbiologica
M Henselová, D Hudecová

Abstract

Seeds of Karwinskia humboldtiana obtained from a 1997 collection in the locality of Villa de Gracía Nuevo (León, Mexico) were contaminated with spores of filamentous fungi, bacteria and yeasts. The concentration of microorganisms in unscarified seeds ranged from 3.0 x 10(3) to 7.5 x 10(3) CFU/g. Predominant were bacterial isolates of the genera Aeromonas sp., Bacillus, and Pseudomonas; from filamentous fungi were identified Alternaria, Aspergillus niger, Cladosporium sp., Fusarium sp., Mucor sp., Penicillium commune, Trichothecium sp.; from yeasts Rhodotorula sp. and Saccharomyces cerevisiae. Seed scarification significantly reduced the microbial contamination. Of the original fungal isolates, only two were identified on scarified seeds, viz. Cladosporium sp. and Saccharomyces cerevisiae; although a relatively high incidence of a unidentifiable of Penicillium sp. was found, the bacterial spectrum was not altered. Treatment of scarified seeds with Vitavax 200 WP and Pomarsol Forte 80 WP (3 mg/g seeds) augmented germination by 10-19% compared to treated unscarified seeds, and by 16-31% compared to untreated unscarified seeds.

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Citations

Aug 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Irene StefaniniDuccio Cavalieri

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