Differences in the Plasma Proteome of Patients with Hypothyroidism before and after Thyroid Hormone Replacement: A Proteomic Analysis

International Journal of Molecular Sciences
Assim A AlfaddaAishah A Ekhzaimy

Abstract

Thyroid hormone is a potent stimulator of metabolism, playing a critical role in regulating energy expenditure and in key physiological mechanisms, such as growth and development. Although administration of thyroid hormone in the form of levo thyroxine (l-thyroxine) has been used to treat hypothyroidism for many years, the precise molecular basis of its physiological actions remains uncertain. Our objective was to define the changes in circulating protein levels that characterize alterations in thyroid hormone status. To do this, an integrated untargeted proteomic approach with network analysis was used. This study included 10 age-matched subjects with newly diagnosed overt hypothyroidism. Blood was collected from subjects at baseline and at intervals post-treatment with l-thyroxine until they reached to euthyroid levels. Plasma protein levels were compared by two-dimensional difference in gel electrophoresis (2D-DIGE) pre- and post-treatment. Twenty differentially expressed protein spots were detected. Thirteen were identified, and were found to be unique protein sequences by MALDI-TOF mass spectrometry. Ten proteins were more abundant in the hypothyroid vs. euthyroid state: complement C2, serotransferrin, complement C3, Ig κ ...Continue Reading

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Citations

May 5, 2018·The Journal of Endocrinology·Maik PietznerNele Friedrich
Jun 16, 2019·International Journal of Molecular Sciences·Daniela Grimm
May 8, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bassam H SabhaElrashdy M Redwan
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May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hicham BenabdelkamelAssim A Alfadda
Aug 7, 2021·Scientific Reports·Barbara Lisowska-MyjakEwa Skarżyńska

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

UNIPROT
Progenesis Same Spots
Flex Analysis
IPA
Mascot
view Q
Progenesis
[UNK]

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