PMID: 8938659Nov 1, 1996Paper

Differences in the prejunctional effects of methacholine and pilocarpine on the release of endogenous acetylcholine from guinea-pig trachea

Naunyn-Schmiedeberg's Archives of Pharmacology
R E ten BergeJ Zaagsma

Abstract

We investigated the effects of the full muscarinic acetylcholine receptor agonist methacholine and the partial and putatively M2-selective agonist pilocarpine on endogenous acetylcholine release from guinea-pig trachea by use of high-performance liquid chromatography with electrochemical detection. Atropine-induced increases in acetylcholine release were used to monitor the system. Electrical field stimulation (8 V, 30 Hz, 0.5 ms for 5 min)-induced acetylcholine release in the presence of neostigmine, with or without preincubation with choline to maximally enhance acetylcholine output, was increased to about 225% by 0.3 microM atropine, indicating functional autoinhibition. However, methacholine (10 microM) did not affect the acetylcholine release, whereas it was enhanced to 166% by 30 microM pilocarpine. When electrical field stimulation was applied at lower intensity (8 V, 16 Hz, 0.1 ms for 5 min) and in the absence of neostigmine, and increase by 0.3 microM atropine (to 177%) but a decrease of the acetylcholine release by 10 microM methacholine (to 65%) and 30 microM pilocarpine (to 63%) were observed. These results clearly demonstrate (i) that inhibition of evoked endogenous acetylcholine release from prejunctional nerve te...Continue Reading

Citations

Oct 3, 2006·European Journal of Pharmacology·Lynette FernandesRoy Goldie
Sep 2, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Deborah A SawatzkyRichard W Costello
Mar 10, 1999·The American Journal of Physiology·J R de HaasJ Zaagsma

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