Differences in the proteome of high-grade versus low-grade canine cutaneous mast cell tumours

The Veterinary Journal
P SchliebenRobert Klopfleisch

Abstract

Cutaneous mast cell tumours (MCTs) are the most common skin tumours in dogs. However, the molecular differences between benign tumours with a good prognosis and highly malignant, invasive and metastatic tumours with short survival times are for the most part unclear. In the present study the proteome of low-grade MCTs with a good prognosis was compared with that of poor-prognosis high-grade tumours independent of their mutational status of exon 11 of the KIT gene. Using two-dimensional difference gel electrophoresis and mass spectrometry, 13 proteins with a significant differential expression between the two groups were identified. Four stress response proteins (HSPA9, PDIA3, TCP1A and TCP1E) were significantly up-regulated in high-grade tumours, while proteins mainly associated with cell motility and metastasis had either increased (WDR1, ACTR3, ANXA6) or decreased (ANXA2, ACTB) expression levels. High-grade tumours also had a paradox down-regulation of transferrin, a protein that is usually up-regulated in neoplastic cells. The histologically observable dedifferentiation of high-grade tumours was reflected by decreased tryptase protein expression levels. Results of quantitative real-time RT-PCR analysis indicated that the dif...Continue Reading

References

Dec 21, 2002·American Journal of Veterinary Research·Sue DowningCheryl A London
Apr 1, 2004·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Cameron L R JonesCheryl A London
Jun 1, 2005·Molecular and Cellular Biology·Xiaoqi LiuRaymond L Erikson
Apr 14, 2006·Neoplasia : an International Journal for Oncology Research·Joshua D WebsterMatti Kiupel
Nov 6, 2007·Annals of the New York Academy of Sciences·Michael Sherman, Gabriele Multhoff
Apr 17, 2008·Cell Cycle·Emma L DavenportFaith E Davies
Jul 23, 2008·Molecular Cancer Research : MCR·Sébastien LetardPatrice Dubreuil
Aug 19, 2008·Journal of the Royal Society, Interface·Gabriel M Altschuler, Keith R Willison
Nov 5, 2008·Veterinary Dermatology·Monika M WelleSilvia Rüfenacht
May 19, 2009·Journal of Comparative Pathology·G Kastengren FröbergK Nordlind
Oct 12, 2010·Journal of Proteome Research·Robert KlopfleischAchim D Gruber
Nov 11, 2010·Veterinary and Comparative Oncology·F TaylorT Scase

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Citations

Feb 6, 2016·The Veterinary Journal·Joanna S Morris
Jan 19, 2016·Journal of Proteomics·Mónica FernandesMarlene Barros
Jan 17, 2016·Translational Research : the Journal of Laboratory and Clinical Medicine·Cristiana CaorsiFrancesco Novelli
Sep 1, 2012·Journal of Comparative Pathology·P SchliebenR Klopfleisch
Nov 8, 2014·Journal of Ovarian Research·Ali BahramiMehdi Pourzaer
Feb 3, 2015·The Veterinary Journal·R Klopfleisch
Jun 28, 2016·Molecular BioSystems·F CecilianiC Lecchi
Feb 15, 2018·International Journal of Molecular Sciences·Katarzyna Zabielska-KoczywąsRoman Lechowski
Oct 28, 2019·Veterinary Medicine and Science·Vanessa S TamlinAnne E Peaston
Feb 6, 2019·TheScientificWorldJournal·Matteo CerquetellaSilvia Vincenzetti

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