Differences in the roles of the two surface-exposed tyrosine residues, Y240 and Y481, of Serratia marcescens chitinase B during processive degradation of crystalline chitin

The Journal of General and Applied Microbiology
Hayuki SugimotoTakeshi Watanabe

Abstract

Chitinase B from Serratia marcescens 2170 is one of the processive chitinases, and it has a linear path of aromatic amino acid residues on the surface and in the catalytic cleft. There are four surface-exposed residues lined-up towards the cleft, Y481, W479, W252, and Y240. The substitution of these residues with alanine causes a decrease in both the extent of the substrate binding and the hydrolytic activity (Katouno et al., 2004). Here, we examine the three mutants without losing the substrate-binding ability, Y240W, Y481W, and Y240W/Y481W. These mutants were prepared for a detailed analysis of the functions of Y240 and Y481, which showed a lower contribution to substrate binding than W479 and W252. The parameters for the binding of the three mutants to crystalline β-chitin were similar to those for the wild type. The hydrolytic activity of Y240W and Y240W/Y481W against crystalline β-chitin was significantly decreased. However, the hydrolytic activity of Y481W was similar to that of the wild type, indicating some differences in the roles of Y240 and Y481 during the processive degradation of crystalline β-chitin. Taken together with the previous results, it was suggested that while Y240 and Y481 were required for the substrate...Continue Reading

References

May 18, 1981·Carbohydrate Research·H Yamada, T Imoto
Nov 1, 1995·Protein Science : a Publication of the Protein Society·C N PaceT Gray
Nov 26, 1997·Journal of Bacteriology·T WatanabeM Regue
Mar 21, 1998·Bioscience, Biotechnology, and Biochemistry·K SuzukiT Watanabe
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·D M van AaltenR K Wierenga
Aug 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·D M van AaltenV G Eijsink
Jan 30, 2003·Acta Crystallographica. Section D, Biological Crystallography·Yannis PapanikolauKyriacos Petratos
Jul 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Gennady ZolotnitskyYuval Shoham
Nov 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Svein J HornVincent G H Eijsink
Oct 4, 2011·The Journal of Biological Chemistry·Christina M PayneGregg T Beckham
Nov 26, 2013·Nucleic Acids Research·Vincent LombardBernard Henrissat
May 28, 2014·Current Opinion in Biotechnology·Gregg T BeckhamChristina M Payne
Jun 5, 2014·Nature Communications·Kiyohiko IgarashiMasahiro Samejima

❮ Previous
Next ❯

Citations

Mar 30, 2017·Biochimica Et Biophysica Acta. General Subjects·Kristýna Slámová, Pavla Bojarová

❮ Previous
Next ❯

Related Concepts

Related Feeds

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.