PMID: 6168710Oct 1, 1981Paper

Differences of cell surface label distribution and redistribution patterns between mammalian keratinocytes and melanocytes in culture

The Journal of Investigative Dermatology
G SchulerP Fritsch

Abstract

Primary guinea pig epidermal cell cultures were subjected to a variety of ultrastructural surface labeling techniques specific for lectin binding sites (Concanavalin A-horseradish peroxidase, wheatgerm agglutinin-chitobiosyl-horseradish peroxidase) or anionic surface sites (Ruthenium red, cationized ferritin). All these techniques were carried out on fixed cells; with cationized ferritin, labeling was also performed on unfixed, viable cells by incubation at 4 degrees C and 37 degrees C for various time periods. Lectin labeling resulted in a diffuse pattern identical for both melanocytes and keratinocytes. Different patterns were obtained with the markers for anionic sites: with both ruthenium red and cationized ferritin, fixed keratinocytes were more heavily labeled than melanocytes, the label being diffuse with randomly distributed globular condensations. Melanocytes, in contrast, were lined by a thin uniform band-like label. On viable keratinocytes, exposed to cationized ferritin at 4 degrees C, the label was confined to randomly disseminated patches which most probably represent the "inherent" distribution of anionic surface sites. At 37 degrees C, this pattern was progressively changed by cluster formation as expression of ...Continue Reading

Citations

Jan 1, 1990·Archives of Dermatological Research·P Nau, G Schaumburg-Lever
Jan 1, 1983·Archives of Dermatological Research·N RomaniP Fritsch
Apr 1, 1983·The Journal of Investigative Dermatology·N RomaniP Fritsch
Jan 1, 1985·The Journal of Investigative Dermatology·M Manabe, H Ogawa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.