PMID: 8601575Jan 1, 1996Paper

Differences of reactivity to interferon gamma in HeLa and CaSki cells: a combined immunocytochemical and flow-cytometric study

Journal of Cancer Research and Clinical Oncology
G LizardD Schmitt

Abstract

We characterized the changes induced by treatment for 48 h with 100 U/ ml interferon gamma (IFN-gamma) on HeLa and CaSki cells, derived from human uterine carcinomas and containing human papillomavirus (HPV) type 16 and HPV type 18 respectively, by studying cell growth, cell morphology, the cell cycle and expression of epidermal growth factor (EGF) receptor, filaggrin-profilaggrin and MHC class II antigen, HLA-DR. The response of the two cell lines to IFN gamma differed in some cases. In both cell lines, the cells remained viable; cell growth was similarly inhibited as shown by cell counts. Signs of morphological changes were essentially observed in HeLa cells. The cell cycle phases, analyzed by flow cytometry were more disturbed in CaSki than in HeLa cells; the proportion of CaSki cells in S phase increased and those in G2 + M decreased. Expression of EGF receptors related to proliferation increased only in CaSki cells while expression of filaggrin-profilaggrin, a marker of differentiation, and HLA-DR, a marker of epithelial cell immune response, was enhanced in both cell lines. The presence of filaggrin-profilaggrin being unexpected in these cells, the specificity of the reaction with the monoclonal antibody AKH1 was confirme...Continue Reading

References

Jul 31, 1990·Biochemical and Biophysical Research Communications·A NawaY Tomoda
May 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J RidgeE H Chang
Sep 1, 1991·American Journal of Obstetrics and Gynecology·R C McGlennenA J Faras
Feb 1, 1991·Journal of Interferon Research·A ChakravarthyA W Hamburger
Jul 1, 1990·The Journal of Investigative Dermatology·A ReanoJ Thivolet
Jan 1, 1986·Ultrastructural Pathology·B J NickoloffV Morhenn
Feb 1, 1986·The Journal of Investigative Dermatology·S A Smith, B A Dale
Jan 1, 1988·Archives of Gynecology and Obstetrics·M CintorinoK Syrjänen
Dec 1, 1987·Proceedings of the Society for Experimental Biology and Medicine·E H ChangJ B Harford
Dec 1, 1985·The Journal of Investigative Dermatology·P FleckmanK A Holbrook
Aug 1, 1984·The Journal of Investigative Dermatology·T Y BashamV B Morhenn
Jan 1, 1993·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·S S GlewP L Stern
Feb 1, 1994·International Journal of Cancer. Journal International Du Cancer·N Coleman, M A Stanley
Jan 1, 1993·Annual Review of Immunology·M A Farrar, R D Schreiber

❮ Previous
Next ❯

Citations

May 10, 2006·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·H-S JinW-S Ahn
Jun 1, 2006·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Yong-Seok LeeWoong-Shick Ahn
Mar 17, 2007·FEBS Letters·Elena BurovaNikolai Nikolsky
Jan 16, 2016·Proteomics. Clinical Applications·Charles T SpencerSebastian Joyce

❮ Previous
Next ❯

Related Concepts

Related Feeds

Related Papers

International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society
W S AhnY-W Kim
European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)
T G HuangH Y Ngan
Proceedings of the National Academy of Sciences of the United States of America
L BuckbinderN Kley
© 2021 Meta ULC. All rights reserved