PMID: 8937800Nov 1, 1996Paper

Different [3H]ouabain binding characteristics of fast and slow skeletal muscles in IDDM and NIDDM diabetic rats

Clinical Physiology
T Bányász, T Kovács

Abstract

The aim of this study was to determine the ouabain receptor density, sodium content and contractile properties of skeletal muscles in rats with insulin-dependent (IDDM) and non-insulin-dependent (NIDDM) diabetes mellitus induced by streptozotocin treatment. These parameters were compared in isolated soleus (SOL) and extensor digitorum longus (EDL) muscles of diabetic animals and their age-matched controls. Reversibility of the diabetes-induced changes was studied by insulin or thyroxine substitution. In IDDM SOL muscles both the maximum [3H]ouabain binding capacity (Bmax) and dissociation constant (Kd) were decreased, the sodium content of the muscles increased and the velocity of contraction and relaxation decreased. Similar changes (except for the reduction of Bmax) were observed in diabetic EDL muscles; however, in this case these differences were less prominent. All of these changes were reversed by insulin substitution, whereas thyroxine treatment normalized only the changes in Bmax and velocity of contraction. In contrast to the changes observed in IDDM, NIDDM increased both Kd and Bmax values. Linear correlation was observed between the velocity of contraction or relaxation and the density of [3H]ouabain binding sites in...Continue Reading

References

Mar 1, 1991·The American Journal of Physiology·J R Del CastilloM C Sulbarán-Carrasco
Mar 17, 1986·Life Sciences·A G Tahiliani, J H McNeill
Oct 1, 1986·Pflügers Archiv : European journal of physiology·G M MolnárT Bányász
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Dec 15, 1993·Journal of the Neurological Sciences·E ScarpiniG Scarlato

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Oct 11, 2005·Journal of Feline Medicine and Surgery·Nicola J GrintAvril E Waterman Pearson

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