Different acetonitrile degraders and degrading genes between anaerobic ammonium oxidation and sequencing batch reactor as revealed by stable isotope probing and magnetic-nanoparticle mediated isolation

The Science of the Total Environment
Yujiao SunDayi Zhang

Abstract

Microbial degraders play crucial roles in wastewater treatment processes, but their use is limited as most microbes are yet unculturable. Stable isotope probing (SIP) is a cultivation-independent technique identifying functional-yet-uncultivable microbes in ambient environment, but is unsatisfactory for substrates with low assimilation rate owing to the low isotope incorporation into DNA. In this study, we used acetonitrile as the target low-assimilation chemical in many wastewater treatment plants and attempted to identify the active acetonitrile degraders in the activated sludge, via DNA-SIP and magnetic-nanoparticle mediated isolation (MMI) which is another cultivation-independent approach without the requirement of substrate labeling. The two approaches identified different active acetonitrile degraders in a 3-day short-term anaerobic ammonium oxidation (ANAMMOX). MMI enriched significantly more acetonitrile-degraders than SIP, showing the advantages in identifying the active degraders for low-assimilation substrates. Sequencing batch reactor (SBR, 30-day degradation) helped in more incorporation of 15N-labeled acetonitrile into the active degraders, thus the same acetonitrile-degraders and acetonitrile-degrading genes were...Continue Reading

References

Jun 1, 1976·Applied and Environmental Microbiology·M J DiGeronimo, A D Antoine
Apr 1, 1990·Journal of Industrial Microbiology·K D ChapatwalaJ H Wolfram
Jul 1, 1990·The Journal of Applied Bacteriology·I R Richardson
Feb 24, 2001·Journal of Basic Microbiology·R P RezendeV R Linardi
Sep 6, 2001·Biotechnology and Bioengineering·J J BeunM C van Loosdrecht
Oct 17, 2001·Applied Microbiology and Biotechnology·J C DiasV R Linardi
Oct 31, 2002·Applied and Environmental Microbiology·Mike ManefieldMark J Bailey
Aug 22, 2003·Annals of Tropical Medicine and Parasitology·P ParolaD Raoult
Sep 18, 2003·Journal of the American Chemical Society·Masanari TsujimuraIsao Endo
Nov 25, 2003·Journal of Applied Microbiology·C O'Reilly, P D Turner
Jul 10, 2004·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Chun-Chin WangLi-Jung Chen
Jan 25, 2005·Applied Microbiology and Biotechnology·Raul MuñozBo Mattiasson
May 5, 2005·Applied and Environmental Microbiology·Cédric F V HobelJakob K Kristjánsson
May 12, 2005·Nature Reviews. Microbiology·Marc G Dumont, J Colin Murrell
Sep 27, 2005·Applied Microbiology and Biotechnology·C M KaoS C Chen
Sep 1, 1989·Applied and Environmental Microbiology·M S NawazJ H Wolfram
Jan 1, 1990·Applied and Environmental Microbiology·A J Schoeffield, H N Williams
Mar 7, 2006·Applied and Environmental Microbiology·Peter H Janssen
Apr 16, 2008·Environmental Science & Technology·Tinggang LiF S Wong
May 14, 2008·Annual Review of Microbiology·Matthias Horn
Aug 27, 2009·Research in Microbiology·Daniele Corsaro, Danielle Venditti
Sep 30, 2009·Molecular Phylogenetics and Evolution·Ania M Cutiño-JiménezCarlos F M Menck
Aug 6, 2010·Biotechnology Advances·Shreenath Prasad, Tek Chand Bhalla
Nov 4, 2010·FEMS Microbiology Ecology·Cécile MilitonPierre Peyret
Jan 25, 2011·Microbial Biotechnology·Dayi ZhangWei E Huang
Jan 26, 2011·Environmental Microbiology·Marc G DumontRalf Conrad
Mar 17, 2012·Mass Spectrometry Reviews·Jana SeifertMartin von Bergen
Jun 1, 2012·BMC Bioinformatics·Ye DengJizhong Zhou
Oct 22, 2013·Microbes and Infection·Gilbert Greub
May 13, 2014·International Journal of Systematic and Evolutionary Microbiology·Jae-Chan Lee, Kyung-Sook Whang

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