Different actin affinities of human cardiac essential myosin light chain isoforms

FEBS Letters
I Morano, Hannelore Haase

Abstract

The N terminus of myosin light chain 1 (MLC-1) of skeletal muscle bind to the C terminus of actin. We investigated whether the N termini of human cardiac MLC-1 isoforms likewise bind to actin. Furthermore, we investigated whether the N-terminal sequence 5-15 (P5-14) of MLC-1 of human atrium (ALC-1) and ventricle (VLC-1) bind with different affinities to actin. Affinity beads were produced by covalently coupling a synthetic peptide corresponding to the N-terminal sequence 4-14 of human VLC-1 to aminohexylagarose in order to bind G-actin. We found, that G-actin specifically binds to the affinity beads. Furthermore, preincubation of G-actin with P5-14 of both ALC-1 and VLC-1 decreased the amount of G-actin recovered from the affinity beads in a concentration-dependent manner. The half-maximal effective concentrations, however were significantly (p < 0.01) different being 0.32 +/- 0.02 microM and 0.71 +/- 0.02 microM for the VLC-1 and ALC-1 peptide, respectively. The appropriate scrambled peptides were without effect up to 3 microM. These results demonstrate the specific interaction between the N-terminal domains of human cardiac MLC-1 isoforms and actin and reveal different actin affinities of MLC-1 isoforms. Weak binding of ALC-1...Continue Reading

References

Mar 22, 1975·Pflügers Archiv : European journal of physiology·C Delcayre, B Swynghedauw
Jul 10, 1987·Nucleic Acids Research·L J SaezL A Leinwand
Nov 12, 1971·Nature·S Lowey, D Risby
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L A LeinwandB Nadal-Ginard
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·V MahdaviB Nadal-Ginard
May 1, 1995·Circulation Research·I MoranoG Michel
Sep 1, 1995·Journal of Cellular Biochemistry·T CaloviniI Morano
Jul 15, 1996·The Journal of Clinical Investigation·M MoranoI Morano

❮ Previous
Next ❯

Citations

Apr 15, 2006·Pflügers Archiv : European journal of physiology·Oleg AndruchovStefan Galler
Jan 18, 2006·Heart Failure Reviews·Bradley M Palmer
Nov 24, 1999·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·F C Luft
Apr 3, 2007·Molecular Diagnosis & Therapy·Melanie Y White, Jennifer E Van Eyk
Apr 1, 2015·Proceedings of the National Academy of Sciences of the United States of America·Piyali GuhathakurtaDavid D Thomas
Oct 25, 2011·Biochemical and Biophysical Research Communications·D V ShchepkinL V Nikitina
Mar 26, 2016·Cardiovascular Research·Lisa-Mareike ScheidDavid Hassel
Aug 3, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Annette HohausHannelore Haase
Jul 4, 2001·The Journal of Biological Chemistry·A SanbeJ Robbins
Dec 5, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Olga M HernandezDanuta Szczesna-Cordary
Feb 8, 2005·The Journal of Biological Chemistry·Valerie B PatchellBarry A Levine

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.