Different activities of the reovirus FAST proteins and influenza hemagglutinin in cell-cell fusion assays and in response to membrane curvature agents

Virology
Eileen K ClancyRoy Duncan

Abstract

The reovirus fusion-associated small transmembrane (FAST) proteins evolved to induce cell-cell, rather than virus-cell, membrane fusion. It is unclear whether the FAST protein fusion reaction proceeds in the same manner as the enveloped virus fusion proteins. We now show that fluorescence-based cell-cell and cell-RBC hemifusion assays are unsuited for detecting lipid mixing in the absence of content mixing during FAST protein-mediated membrane fusion. Furthermore, membrane curvature agents that inhibit hemifusion or promote pore formation mediated by influenza hemagglutinin had no effect on p14-induced cell-cell fusion, even under conditions of limiting p14 concentrations. Standard assays used to detect fusion intermediates induced by enveloped virus fusion proteins are therefore not applicable to the FAST proteins. These results suggest the possibility that the nature of the fusion intermediates or the mechanisms used to transit through the various stages of the fusion reaction may differ between these distinct classes of viral fusogens.

References

Dec 8, 1995·The Journal of Biological Chemistry·S Günther-AusbornT Stegmann
May 1, 1995·Journal of Virology·L ChernomordikJ Zimmerberg
Feb 22, 1994·Biochemistry·P L YeagleT D Flanagan
Jul 15, 1996·Chemistry and Physics of Lipids·L Chernomordik
Jul 11, 2000·Molecular Biology of the Cell·E Leikina, L V Chernomordik
Aug 10, 2000·The Journal of Cell Biology·Y Gaudin
Oct 18, 2002·Current Opinion in Cell Biology·Reinhard Jahn, Helmut Grubmüller
Mar 13, 2003·Molecular Biology of the Cell·Ruben M MarkosyanGrigory B Melikyan
Aug 29, 2003·Biophysical Journal·Aditya MittalJoe Bentz
Sep 2, 2003·Current Opinion in Structural Biology·Lukas K TammVolker Kiessling
Oct 7, 2003·Annual Review of Biochemistry·Leonid V Chernomordik, Michael M Kozlov
Dec 13, 2003·Journal of Virology·Tatiana ZavorotinskayaLorraine M Albritton
Feb 18, 2004·Virology·Roy DuncanDon Stoltz
Sep 16, 2004·The Journal of Membrane Biology·F S Cohen, G B Melikyan
Jan 15, 2005·Journal of Virology·LiTing T ChengRichard W Compans
Apr 6, 2005·The Journal of Cell Biology·Elena ZaitsevaLeonid V Chernomordik
Apr 12, 2005·Nature Structural & Molecular Biology·Yibin XuYeon-Kyun Shin
Oct 1, 2005·Molecular Biology of the Cell·Ruben M MarkosyanGrigory B Melikyan
Nov 5, 2005·Cell·Leonid V Chernomordik, Michael M Kozlov
Dec 17, 2005·Nature Reviews. Microbiology·Margaret Kielian, Félix A Rey
Dec 21, 2005·The Journal of Cell Biology·Christoph Reese, Andreas Mayer
Aug 17, 2006·Nature Reviews. Molecular Cell Biology·Reinhard Jahn, Richard H Scheller
Oct 3, 2006·Developmental Cell·Benjamin PodbilewiczLeonid V Chernomordik
Oct 4, 2006·EMBO Reports·Félix A Rey
Feb 10, 2007·Science·Stéphane RocheStéphane Bressanelli

❮ Previous
Next ❯

Citations

Sep 24, 2014·Trends in Microbiology·Marta Ciechonska, Roy Duncan
Dec 6, 2014·Journal of Biological Engineering·Elise CachatJamie A Davies
Aug 5, 2011·Journal of Virology·Ryan S NoyceKaren L Mossman
Apr 27, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Meital Oren-Suissa, Benjamin Podbilewicz
Aug 1, 2020·Frontiers in Bioengineering and Biotechnology·Erika SzymanskiIonat Zurr

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Antipsychotic Drugs

Antipsychotic drugs are a class of medication primarily used to manage psychosis (including delusions, hallucinations, paranoia or disordered thought), principally in schizophrenia and bipolar disorder. Discover the latest research on antipsychotic drugs here