Different adaptive strategies in E. coli populations evolving under macronutrient limitation and metal ion limitation

BMC Evolutionary Biology
Omar M WarsiD E Dykhuizen

Abstract

Adaptive responses to nutrient limitation involve mutations that increase the efficiency of usage or uptake of the limiting nutrient. However, starvation of different nutrients has contrasting effects on physiology, resulting in different evolutionary responses. Most studies performed to understand these evolutionary responses have focused only on macronutrient limitation. Hence our understanding of adaptation under limitation of other forms of nutrients is limited. In this study, we compared the evolutionary response in populations evolving under growth-limiting conditions for a macronutrient and a major cation. We evolved eight populations of E. coli in nutrient-limited chemostats for 400 generations to identify the genetic basis of the mechanisms involved in efficient usage of two nutrients: nitrogen and magnesium. Our population genomic sequencing work, based on this study and previous work, allowed us to identify targets of selection under these nutrient limiting conditions. Global transcriptional regulators glnGL were targets of selection under nitrogen starvation, while proteins involved in outer-membrane biogenesis (genes from the lpt operon) were targets of selection under magnesium starvation. The protein involved in ...Continue Reading

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Citations

Jan 25, 2020·Molecular Biology and Evolution·Omar WarsiDan I Andersson
Sep 1, 2020·Probiotics and Antimicrobial Proteins·Sofia Magalhães MoreiraHilário Cuquetto Mantovani

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Methods Mentioned

BETA
PCR
Bioscreen

Software Mentioned

breseq
Geneious

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