Different alpha crystallin expression in human age-related and congenital cataract lens epithelium

BMC Ophthalmology
Jing YangJianjun Gu

Abstract

The purpose of this study was to investigate the different expressions of αA-crystallin and αB-crystallin in human lens epithelium of age-related and congenital cataracts. The central part of the human anterior lens capsule approximately 5 mm in diameter together with the adhering epithelial cells, were harvested and processed within 6 hours after cataract surgery from age-related and congenital cataract patients or from normal eyes of fresh cadavers. The mRNA and soluble protein levels of αA-crystallin and αB-crystallin in the human lens epithelium were detected by real-time PCR and western blots, respectively. The mRNA and soluble protein expressions of αA-crystallin and αB-crystallin in the lens epithelium were both reduced in age-related and congenital cataract groups when compared with the normal control group. However, the degree of α-crystallin loss in the lens epithelium was highly correlated with different cataract types. The α-crystallin expression of the lens epithelium was greatly reduced in the congenital cataract group but only moderately decreased in the age-related cataract group. The reduction of αA-crystallin soluble protein levels in the congenital cataract group was approximately 2.4 fold decrease compared w...Continue Reading

References

Dec 1, 1996·Experimental Eye Research·J A CarverR J Truscott
Mar 7, 2002·Experimental Eye Research·Steven Bassnett
Mar 23, 2002·Progress in Molecular and Subcellular Biology·Roy Quinlan
Feb 5, 2003·Experimental Eye Research·Joseph Horwitz
Aug 11, 2004·Progress in Biophysics and Molecular Biology·Hans BloemendalAnnette Tardieu
Sep 29, 2004·Investigative Ophthalmology & Visual Science·Xiaohui WangDavid C Beebe
Dec 15, 2006·Progress in Retinal and Eye Research·Usha P Andley
Dec 21, 2007·The International Journal of Biochemistry & Cell Biology·Usha P Andley
May 26, 2009·Biochimica Et Biophysica Acta·K Krishna Sharma, Puttur Santhoshkumar
Oct 5, 2010·The International Journal of Biochemistry & Cell Biology·G Martinez, R U de Iongh
Jun 22, 2012·Acta Ophthalmologica·Elena ProkofyevaEberhart Zrenner
Jul 10, 2012·Ophthalmology·Grace M RichterUNKNOWN Los Angeles Latino Eye Study Group
Nov 8, 2012·Investigative Ophthalmology & Visual Science·Wenyong HuangMingguang He
Dec 3, 2014·Biochimica Et Biophysica Acta·Hiroaki SakaueNoriko Fujii

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

GraphPad
Prism
Image J

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.