PMID: 2108129Apr 1, 1990Paper

Different and rapid responses of four cyanobacterial psbA transcripts to changes in light intensity

Journal of Bacteriology
S A BustosS S Golden

Abstract

The genome of the cyanobacterium Synechococcus sp. strain PCC 7942 contains three psbA genes which encode two forms of the D1 protein of photosystem II. Experiments using psbA-lacZ translational fusions and Western blot (immunoblot) analysis have shown that the psbA genes respond differently to changes in light intensity, altering the ratio of the two forms of D1 in the thylakoid membrane. Each gene produces a 1.2-kilobase (kb) mRNA. A probe specific for psbAII transcripts also identified a 1.6-kb mRNA which starts 419 base pairs upstream of the 5' end of the 1.2-kb species and overlaps the entire 1.2-kb transcript. This 419-base-pair region includes an open reading frame (ORF1) of 114 amino acids. We investigated the effects of changes in light intensity on psbAII transcript levels in a series of light shift experiments in the wild-type Synechococcus sp. and in AMC084, a mutant which does not produce the 1.6-kb transcript. After exposure to high light intensities for 15 min, the level of the 1.2-kb psbAII transcript increased in both strains. This transcript was not detected in either strain after transfer to low light intensity. The psbAIII transcript showed the same pattern of response as the 1.2-kb psbAII transcript, wherea...Continue Reading

References

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Citations

Aug 1, 2009·Cellular and Molecular Life Sciences : CMLS·Paula MuloEva-Mari Aro
Apr 1, 1993·Molecular & General Genetics : MGG·A MohamedC Jansson
Dec 29, 2012·The Journal of Biological Chemistry·David J VinyardG Charles Dismukes
Feb 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·D CampbellA K Clarke
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·T Trebitsh, A Danon
Jan 1, 1992·DNA Sequence : the Journal of DNA Sequencing and Mapping·B Séraphin
Aug 19, 2008·Photosynthesis Research·Marvin Edelman, Autar K Mattoo
May 8, 2007·Applied and Environmental Microbiology·Oliver KilianArthur R Grossman
Mar 29, 2001·Journal of Bacteriology·T Mori, C H Johnson
Jun 3, 2009·Microbiology and Molecular Biology Reviews : MMBR·D J ScanlanF Partensky
Nov 4, 1998·Archives of Biochemistry and Biophysics·J M García-FernándezF Partensky
Apr 12, 1996·Biochemical Pharmacology·M H Melner
Apr 1, 1995·Journal of Bacteriology·S S Golden
Mar 1, 1996·Microbiological Reviews·P AlifanoC B Bruni

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