Different antimicrobial susceptibility testing methods to detect ertapenem resistance in Enterobacteriaceae: VITEK2, MicroScan, Etest, disk diffusion, and broth microdilution

Journal of Microbiological Methods
Miae Lee, Hae-Sun Chung

Abstract

We investigated different antimicrobial susceptibility testing methods to detect ertapenem resistance in Enterobacteriaceae. A total of 72 Enterobacteriaceae isolates were collected from a clinical microbiology laboratory of a tertiary university hospital, all of which were detected ertapenem resistance by the VITEK2 system. Bacterial identification and antimicrobial susceptibility were determined using the VITEK2. Ertapenem susceptibility test was performed using the MicroScan, Etest and a disk diffusion test. Ertapenem MICs were confirmed using the broth microdilution (BMD). Sensitivity, specificity, and positive and negative predictive values (PPV and NPV, respectively) of each method for the detection of ertapenem resistance were calculated. Carbapenemases and AmpC β-lactamase were screened using phenotypic methods. Among the 72 isolates, 20 isolates (27.8%) were resistant to ertapenem. Etest showed high sensitivity and specificity (85.0% and 88.5%, respectively) and excellent concordance with BMD. The disk diffusion test had the lowest sensitivity of 50.0%. The VITEK2 showed the lowest essential and categorical agreement (30.5% and 27.8%, respectively). The MicroScan showed relatively good agreement with BMD compared to th...Continue Reading

References

May 17, 2001·Antimicrobial Agents and Chemotherapy·D M LivermoreD L Shungu
May 20, 2004·The Journal of Antimicrobial Chemotherapy·David E NixMark J DiNubile
Mar 11, 2005·Expert Review of Anti-infective Therapy·George G ZhanelDaryl J Hoban
Sep 29, 2005·The Journal of Antimicrobial Chemotherapy·Julie R SamuelPeter Jenkinson
May 10, 2007·Drugs·George G ZhanelJames A Karlowsky
Jul 24, 2010·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M VadingC G Giske
Jul 28, 2010·Antimicrobial Agents and Chemotherapy·Aurora García-FernándezAlessandra Carattoli
Mar 1, 2011·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Jiunn-Jong WuJing-Jou Yan
Mar 28, 2012·Journal of Pain & Palliative Care Pharmacotherapy·UNKNOWN Centers for Disease Control and Prevention, Public Health Service, U.S. Department Health
Jan 8, 2013·Diagnostic Microbiology and Infectious Disease·David van DuinRobert A Bonomo
Nov 5, 2013·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Hélène PailhorièsMatthieu Eveillard

❮ Previous
Next ❯

Citations

Mar 15, 2016·Journal of Microbiological Methods·Fabiane Lacerda FranciscoFelipe Rebello Lourenço
Apr 5, 2016·Expert Review of Molecular Diagnostics·Yasufumi Matsumura, Johann D Pitout
Oct 23, 2016·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·A Aguirre-Quiñonero, L Martínez-Martínez
Sep 1, 2017·Clinical Microbiology Reviews·Scott QuainooHeiman F L Wertheim
Sep 19, 2019·Antimicrobial Agents and Chemotherapy·Yune-Fang UengYee-Yung Ng
Sep 13, 2017·BMC Infectious Diseases·Birkneh Tilahun TadesseSabine Dittrich

❮ Previous
Next ❯

Related Concepts

Related Feeds

Absence Epilepsy

Absence epilepsy is a common seizure disorder in children which can produce chronic psychosocial sequelae. Discover the latest research on absence epilepsies here.

Carbapenems (ASM)

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.

Carbapenems

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.