Different behavior of meniscal cells in collagen II/I,III and Hyaff-11 scaffolds in vitro

Tissue Engineering. Part a
Catharina ChiariStefan Nehrer

Abstract

The purpose of this study was to evaluate the behavior of ovine meniscal cells seeded on biomaterials made from collagen and hyaluronan, respectively. Ovine meniscal cells were isolated from the medial menisci of stifle joints, expanded in monolayer culture, and seeded on scaffolds made of collagen type II and I/III and a hyaluronan derivative (Hyaff-11). The samples were cultured for 12 h and 7, 14, 21, and 28 days. Histological analysis, electron microscopy, biochemical assays for glycosaminoglycans (GAGs) and DNA, and reverse transcriptase polymerase chain reaction analysis for collagens were performed. The cells attached well to both biomaterials and produced tissue-specific proteins, such as GAG and collagen type I, over a period of 28 days. Differences between the biomaterials were seen with respect to cell distribution, cell morphology, and the dynamics of GAG synthesis. The results show that ovine meniscal cells express their phenotype in both biomaterials. In terms of biology, collagen and hyaluronan are both suitable for tissue engineering in meniscal regeneration. It remains to be determined which scaffold possesses adequate biomechanical properties for successful in vivo application.

References

Aug 1, 1978·The Journal of Pathology·F N GhadiallyK L Massey
Aug 1, 1992·Journal of Clinical Periodontology·D Quteish, A E Dolby
Feb 1, 1990·Journal of Biomedical Materials Research·H PetiteD Herbage
May 1, 1983·The American Journal of Sports Medicine·S P Arnoczky, R F Warren
Nov 1, 1995·Journal of Biomedical Materials Research·K S WeadockM G Dunn
Oct 23, 1997·The Journal of Bone and Joint Surgery. British Volume·S R FrenkelJ M Pachence
Jan 12, 1999·Biomaterials·D CampocciaD F Williams
Apr 30, 1999·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·L A SolchagaA I Caplan
Jul 9, 1999·Journal of Biomedical Materials Research·P BrunR Cortivo
Oct 18, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Douglas J WylandThomas P Vail
Dec 31, 2003·Biomaterials·P BumaR P H Veth
Sep 24, 2004·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ronald DorotkaStefan Nehrer
Mar 1, 2005·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Eric M Darling, Kyriacos A Athanasiou
May 14, 2005·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·J Richard Steadman, William G Rodkey
Jun 3, 2005·Clinical Orthopaedics and Related Research·Maurilio MarcacciStefano Zanasi
Jun 28, 2005·Osteoarthritis and Cartilage·P C M VerdonkG Verbruggen
Oct 11, 2005·Archives of Orthopaedic and Trauma Surgery·V MartinekA B Imhoff
May 30, 2006·Osteoarthritis and Cartilage·C ChiariS Nehrer
Jul 13, 2006·The American Journal of Sports Medicine·Alberto GobbiMaurilio Marcacci
Dec 1, 2006·Sports Medicine and Arthroscopy Review·Gwendolyn M Hoben, Kyriacos A Athanasiou
Mar 21, 2007·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Maurilio MarcacciAnthony P Hollander
May 9, 2007·Tissue Engineering·Gwendolyn M HobenKyriacos A Athanasiou

❮ Previous
Next ❯

Citations

Mar 11, 2009·Biotechnology and Bioengineering·Najmuddin J Gunja, Kyriacos A Athanasiou
Jul 11, 2009·Journal of Biomedical Materials Research. Part a·Catherine Gérard, Charles J Doillon
Mar 12, 2010·Lab on a Chip·Nina TandonGordana Vunjak-Novakovic
May 29, 2018·Scientific Reports·Solaiman TarafderChang H Lee
Jul 1, 2011·Cartilage·Henning MadryMagali Cucchiarini
Mar 19, 2009·Tissue Engineering. Part a·Bunsho KaoYoshiaki Hosaka
Jul 23, 2015·Stem Cells International·Weimin GuoQuanyi Guo
Sep 18, 2020·Tissue Engineering. Part a·Hoda A ElkhenanyAdetola B Adesida

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved