PMID: 8609159Apr 1, 1996Paper

Different biosynthetic transport routes to the plasma membrane in BHK and CHO cells

The Journal of Cell Biology
T YoshimoriK Simons

Abstract

The question of how membrane proteins are delivered from the TGN to the cell surface in fibroblasts has received little attention. In this paper we have studied how their post-Golgi delivery routes compare with those in epithelia] cells. We have analyzed the transport of the vesicular stomatitis virus G protein, the Semliki Forest virus spike glycoprotein, both basolateral in MDCK cells, and the influenza virus hemagglutinin, apical in MDCK cells. In addition, we also have studied the transport of a hemagglutinin mutant (Cys543Tyr) which is basolateral in MDCK cells. Aluminum fluoride, a general activator of heterotrimeric G proteins, inhibited the transport of the basolateral cognate proteins, as well as of the hemagglutinin mutant, from the TGN to the cell surface in BHK and CHO cells, while having no effect on the surface delivery of the wild-type hemagglutinin. Only wild-type hemagglutinin became insoluble in the detergent CHAPS during transport through the BHK and CHO Golgi complexes, whereas the basolateral marker proteins remained CHAPS-soluble. We also have developed an in vitro assay using streptolysin O-permeabilized BHK cells, similar to the one we have previously used for analyzing polarized transport in MDCK cells ...Continue Reading

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Citations

Sep 12, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·N T Ktistakis
Sep 21, 2000·Journal of Cellular Biochemistry·N CaillolD Lombardo
Sep 28, 1998·Matrix Biology : Journal of the International Society for Matrix Biology·E C Davis, R P Mecham
Feb 13, 2001·Current Opinion in Cell Biology·J Taunton
Aug 1, 1997·Current Opinion in Cell Biology·L M Traub, S Kornfeld
Aug 1, 1997·Current Opinion in Cell Biology·T Harder, K Simons
Feb 7, 1998·Current Opinion in Neurobiology·D HigginsG Banker
Mar 6, 1999·Current Opinion in Plant Biology·K Matsuoka, S Y Bednarek
Jul 4, 2001·Nature Reviews. Molecular Cell Biology·H SprongG van Meer
Jan 27, 2004·Nature Cell Biology·Charles YeamanVivek Malhotra
Mar 2, 2005·Nature Reviews. Molecular Cell Biology·Enrique Rodriguez-BoulanAnne Müsch
Feb 4, 2005·Molecular Biology of the Cell·John G Lock, Jennifer L Stow
Feb 16, 2002·The EMBO Journal·Sangiliyandi GurunathanJeffrey E Gerst
Apr 23, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·F Valtorta, C Leoni
Jan 13, 2010·Cold Spring Harbor Perspectives in Biology·W James Nelson
Dec 10, 1999·Journal of Virology·S AskovićJ L Portis
Nov 2, 2005·Molecular and Cellular Biology·Aki ManninenKai Simons
Sep 25, 2003·Annual Review of Plant Biology·Christina E OfflerMark J Talbot
Sep 5, 2002·Annual Review of Physiology·Qais Al-Awqati
Mar 19, 2013·Virology Journal·Bishnupriya Bhattacharya, Polly Roy

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