Different calcium channels are coupled to potassium channels with distinct physiological roles in vagal neurons

Proceedings. Biological Sciences
P Sah

Abstract

Whole-cell and sharp microelectrode recordings were obtained from neurons of rat dorsal motor nucleus of the vagus (DMV) in transverse slices of the rat medulla maintained in vitro. Calcium currents were studied with sodium currents blocked with tetrodotoxin, potassium currents blocked by perfusing the cell with caesium as the main cation and using barium as the charge carrier. From a holding potential of -60 mV, inward currents activated at potentials positive of -50 mV and peaked around 0 mV. Voltage clamping the neuron at more hyperpolarised potentials did not reveal any low-threshold inward current. The inward current was effectively blocked by cadmium (100 microM) and nicked (1 mM), suggesting that it is carried by voltage-dependent calcium channels. The inward current could be separated into three pharmacologically distinct components: 40% of the whole cell current was omega-conotoxin sensitive; 20% of the current was nifedipine sensitive; and the rest was blocked by high concentrations of cadmium and nickel. This remaining current cannot be due to P-type calcium channels as omega-agatoxin had no effect on the inward current. Nifedipine had no significant effect on the action potential. Application of omega-conotoxin redu...Continue Reading

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Citations

Dec 23, 1998·Journal of the Autonomic Nervous System·D R IrelandE M McLachlan
May 17, 2002·Progress in Neurobiology·Pankaj Sah, E S Louise Faber
Jan 19, 2000·Progress in Neurobiology·S A Waterman
Jun 15, 2004·Journal of Toxicology and Environmental Health. Part a·Joseph J PancrazioThomas J O'Shaughnessy
Aug 24, 2007·Clinical and Experimental Pharmacology & Physiology·E S Louise Faber, Pankaj Sah
Jul 27, 2012·Journal of Neurophysiology·Matthew D AbbinantiRonald M Harris-Warrick
Jan 27, 2012·Journal of Neurophysiology·Matthew D Abbinanti, Ronald M Harris-Warrick
Nov 4, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Joshua A Goldberg, Charles J Wilson
Sep 29, 2011·Annual Review of Physiology·John P AdelmanPankaj Sah
Nov 6, 2013·The European Journal of Neuroscience·Philippe AlixVincent Seutin
Sep 6, 2000·Clinical and Experimental Pharmacology & Physiology·P Sah, P Davies
Apr 7, 2004·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·E S Louise Faber, Pankaj Sah
Sep 21, 2004·Nature Reviews. Neuroscience·Martin Stocker
Jun 1, 1997·Journal of Neurophysiology·M KobayashiT Morimoto
May 30, 1998·Journal of Neurophysiology·L M Hurley, K Graubard
May 16, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·K N Browning, R A Travagli

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