Jul 26, 2005

Different cellular responses evoked by natural and stoichiometric synthetic chrysotile asbestos

Toxicology and Applied Pharmacology
Elena GazzanoDario Ghigo

Abstract

The carcinogenic potency of asbestos, including chrysotile, is well established. Several physico-chemical features of the fibers appear implied, such as fibrous habit, size, crystallinity, morphology, and surface active metal ions, where free radical generation may take place. In contrast to other asbestos forms, iron is not a stoichiometric component of chrysotile, but is only present together with other extraneous ions as a magnesium- and silicon-replacing contaminant. To determine the role played by contaminating ions and morphological features of the fibers, a stoichiometric chrysotile with constant structure and morphology was synthesized in hydrothermal conditions. Free radical generation and the effects of these fibers on human lung epithelial A549 cells have been compared to that elicited by a well known toxic natural chrysotile (UICC A, from Rhodesia). After a 24-h incubation, the natural, but not the synthetic, form exerted a cytotoxic effect, detected as leakage of lactate dehydrogenase. Homolytic rupture of a C-H bond and lipoperoxidation in A549 cells took place in the presence of the natural, but not of the synthetic, chrysotile. Antioxidant systems were also affected differently. The pentose phosphate pathway and...Continue Reading

  • References24
  • Citations13

References

Mentioned in this Paper

Metabolic Process, Cellular
Thiobarbituric Acid Reactive Substances
PGD gene
Biochemical Pathway
Exertion
Pentose Phosphate Pathway
Glucose-6-phosphate Dehydrogenase Activity
Magnesium Measurement
Lung
Extracellular

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