PMID: 2119808Sep 14, 1990Paper

Different cellular targets for Cu- and Fe-catalyzed oxidation observed using a Cu-compatible thiobarbituric acid assay

Biochimica Et Biophysica Acta
D Gelvan, P Saltman

Abstract

The widely used thiobarbituric acid (TBA) assay for oxidative damage to biomolecules fails in Cu2(+)-containing solutions due to the formation of a cloudy precipitate. The chelation of Cu2+ ions with EDTA or Chelex was investigated. Both prevented precipitate formation, but only Chelex allowed proper color development in the TBA assay. The Chelex modified assay could be adapted to a variety of systems, and was applied to the detection of Cu2+/ascorbate dependent deoxyribose breakdown and oxidative damage in erythrocyte ghosts, lysates and whole cells. Using this method, it was shown that Cu2+/ascorbate caused membrane damage in ghosts but not in whole red blood cells (RBC). Fe3+/ascorbate, on the other hand, caused formation of TBA-reactive products even in whole RBC. When Cu2+ and Fe3+ were presented to isolated hemoglobin as their 1:1 nitrilotriacetate complexes, the protein bound 10-12 cupric ions per molecule, but no ferric ions. It is suggested that oxidative damage catalyzed by copper or iron ions has different cellular targets, determined by the different binding properties of the two metals to various cellular components.

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Citations

Feb 1, 1997·Biological Trace Element Research·D A Clopton, P Saltman
Jan 1, 1996·Free Radical Biology & Medicine·B R Van Dyke, P Saltman
Jul 1, 1996·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·I Fagerheim, E Lingaas
Feb 4, 2011·Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·Bagepalli Srinivas Ashok KumarBachappa Manoj
Feb 19, 2016·Phytotherapy Research : PTR·Ashique ShaikhJagadevappa S Patil
Jan 23, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Shivsharan B DhaddeS Badami
Feb 25, 2011·Chemico-biological Interactions·Ganesh Chandra Jagetia, Tiyagura Koti Reddy
Jan 7, 2011·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Punit BansalM K Unnikrishnan
Jul 21, 2010·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·B S Ashok KumarVeeresh P Veerapur
Jun 7, 2013·The Journal of Pharmacy and Pharmacology·Pawan G NayakKarkala Sreedhara Ranganath Pai
Aug 18, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Ganesh Chandra JagetiaRajendra Kedlaya
Jun 7, 2005·Life Sciences·Ganesh Chandra Jagetia, Tiyyagura Koti Reddy
Jul 30, 2014·International Journal of Pharmaceutics·Madipatla Prathyusha RaoBoreddy Shivanandappa Thippeswamy
Feb 8, 2008·Journal of Inorganic Biochemistry·Rozena Baruch-Suchodolsky, Bilha Fischer
Dec 18, 2013·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Sudhanshu Kumar BhartiAwanish Kumar
Nov 3, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Subhankar BiswasK Sreedhara R Pai
Mar 10, 1999·The American Journal of Physiology·L Mark, E P Ingenito
Sep 13, 2006·International Journal of Radiation Biology·K R PrabhakarM K Unnikrishnan
May 14, 2005·The Journal of Biological Chemistry·Alexander I KuzmenkoFrancis X McCormack
Mar 1, 1992·Journal of Inorganic Biochemistry·M AlberghinaE Rizzarelli
Apr 22, 1992·Biochimica Et Biophysica Acta·D GelvanP Saltman
Nov 23, 2006·Chemico-biological Interactions·K R PrabhakarM K Unnikrishnan

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