Different characteristics of endothelial cells from central and peripheral human cornea in primary culture and after subculture

In Vitro Cellular & Developmental Biology. Animal
J BednarzK Engelmann

Abstract

Several methods for isolation and cultivation of human corneal endothelial cells have been described during the last few decades. In contrast to the situation in vivo, the cultured cells show mitogenic activity but often lose their typical morphological appearance. In this paper, we describe a technique to isolate and cultivate morphologically unchanged endothelium from the human cornea. This method revealed different characteristics of endothelial cells according to their position within the human cornea. Endothelial cells isolated from the central part have a morphology similar to that of cells in vivo (i.e., they are densely packed and show no mitogenic activity). In contrast, endothelial cells derived from the peripheral part of the cornea are characterized by mitogenic activity but their cell-to-cell attachment seems to be less tight than in vivo. The significance of these two different endothelial cell types for wound healing in the human cornea is discussed.

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Citations

Oct 19, 2011·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Yan WangYizhi Liu
Apr 27, 2004·Experimental Eye Research·Katrin EngelmannMonika Valtink
Jul 11, 2003·Progress in Retinal and Eye Research·Nancy C Joyce
Feb 25, 2003·The British Journal of Ophthalmology·G ThuretP Gain
Mar 13, 2013·Regenerative Medicine·Alfonso L SabaterJavier Moreno-Montañés
Sep 18, 2015·Journal of Functional Biomaterials·Jesintha NavaratnamAboulghassem Shahdadfar
Jan 29, 2013·Progress in Retinal and Eye Research·Tatsuya MimuraShiro Amano
Sep 13, 2011·Experimental Eye Research·Nancy C Joyce
Mar 31, 2004·Journal of Cataract and Refractive Surgery·Henry F EdelhauserUNKNOWN ICL in Treatment of Myopia Study Group
Feb 14, 2015·Canadian Journal of Ophthalmology. Journal Canadien D'ophtalmologie·Gregory MoloneyRaj N Devasahayam
Aug 9, 2016·Regenerative Medicine·Matthias FuestJodhbir S Mehta
Apr 13, 2018·Current Eye Research·Ingrida SmeringaiovaKaterina Jirsova
Mar 3, 2017·BioMed Research International·Mariam Lotfy KhaledYutao Liu
Jul 20, 2002·International Ophthalmology Clinics·Arash J Ahmadi, Frederick A Jakobiec
May 6, 2020·Scientific Reports·Ricardo F FraustoAnthony J Aldave
Dec 6, 2020·Stem Cell Research & Therapy·Nicole Ming SieJodhbir S Mehta
Dec 29, 2020·Acta Ophthalmologica·Daniele SpinozziSilke Oellerich
Aug 8, 2017·Survey of Ophthalmology·Bert Van den BogerdNadia Zakaria

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