Different chitin synthase genes are required for various developmental and plant infection processes in the rice blast fungus Magnaporthe oryzae.

PLoS Pathogens
Ling-An KongYou-Liang Peng

Abstract

Chitin is a major component of fungal cell wall and is synthesized by chitin synthases (Chs). Plant pathogenic fungi normally have multiple chitin synthase genes. To determine their roles in development and pathogenesis, we functionally characterized all seven CHS genes in Magnaporthe oryzae. Three of them, CHS1, CHS6, and CHS7, were found to be important for plant infection. While the chs6 mutant was non-pathogenic, the chs1 and chs7 mutants were significantly reduced in virulence. CHS1 plays a specific role in conidiogenesis, an essential step for natural infection cycle. Most of chs1 conidia had no septum and spore tip mucilage. The chs6 mutant was reduced in hyphal growth and conidiation. It failed to penetrate and grow invasively in plant cells. The two MMD-containing chitin synthase genes, CHS5 and CHS6, have a similar expression pattern. Although deletion of CHS5 had no detectable phenotype, the chs5 chs6 double mutant had more severe defects than the chs6 mutant, indicating that they may have overlapping functions in maintaining polarized growth in vegetative and invasive hyphae. Unlike the other CHS genes, CHS7 has a unique function in appressorium formation. Although it was blocked in appressorium formation by germ tu...Continue Reading

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Citations

Jan 11, 2014·Molecular Plant-microbe Interactions : MPMI·Haifeng ZhangZhengguang Zhang
Aug 8, 2015·Environmental Microbiology·Joon-Hee HanKyoung Su Kim
Jan 15, 2015·Molecular Plant Pathology·Yue ChenZhengguang Zhang
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May 4, 2016·Bioscience, Biotechnology, and Biochemistry·Akira YoshimiKeietsu Abe
Apr 19, 2016·Molecular Plant-microbe Interactions : MPMI·Xiliang QiHongmei Cheng
Jul 15, 2017·Molecular & Cellular Proteomics : MCP·Rui-Jin WangYou-Liang Peng
Aug 2, 2014·Nature Communications·Huili ZhangJin-Rong Xu
Mar 13, 2014·Antimicrobial Agents and Chemotherapy·Chantal FernandesTeresa Gonçalves

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Datasets Mentioned

BETA
JF912404
JF912405
JF912406
JF912407
JF912408
JF912409
EU935590

Methods Mentioned

BETA
PCR
phosphotransferase

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