Different counteracting host immune responses to clade 2.2.1.1 and 2.2.1.2 Egyptian H5N1 highly pathogenic avian influenza viruses in naïve and vaccinated chickens

Veterinary Microbiology
Ahmed A SamyTakehiko Saito

Abstract

In Egypt, two distinct lineages of H5N1 highly pathogenic avian influenza (HPAI) viruses, "classic 2.2.1.2" and "variant 2.2.1.1" strains, have evolved. The underlying host immune responses counteracting these viruses in chickens remain not well understood. In the present study, the cytokine responses to a classic strain (C121) and those to a variant strain (V1063) were compared in naïve and vaccinated chickens. In naïve chickens, the C121 replicated more efficiently than the V1063. Both the C121 and the V1063 increased interferon (IFN)-γ and interleukin (IL)-10 gene expression at 48 h post inoculation (hpi) in the lung and spleen but the levels of these cytokines were lower in chickens infected with the C121 than those infected with the V1063. In contrast, in chickens vaccinated with inactivated C121-based vaccine, the C121 replicated less than the V1063. Both challenge with the C121 and that with the V1063 did not increase IFN-γ gene expression at 48 hpi; rather, the C121 increased IL-4 gene expression in the lung accompanied with lower viral titer and higher HI titers. These results suggested that the pathogenicity of HPAI viruses correlated with IFN-γ-producing helper and/or cytotoxic T cell responses in naïve chickens, whe...Continue Reading

References

Jan 29, 2002·Archives of Virology·E HoffmannD R Perez
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 11, 2006·Immunological Reviews·Hirokazu HikonoDavid L Woodland
Jul 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Rachelle SalomonRobert G Webster
Dec 3, 2009·Viral Immunology·Stéphane DavietRima Zoorob
Jan 5, 2011·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Adam J KarpalaAndrew G D Bean
Mar 7, 2012·Microbiology and Molecular Biology Reviews : MMBR·Jennifer R TisoncikMichael G Katze
May 2, 2014·Nucleic Acids Research·Marco BiasiniTorsten Schwede
Nov 6, 2014·Molecular Biology and Evolution·Lam-Tung NguyenBui Quang Minh

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Citations

Apr 14, 2016·Veterinary Microbiology·Kevin Yana NjaboThomas B Smith
Dec 17, 2019·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Mohammed Ismail HassanSerageldeen Sultan
Mar 1, 2019·Infection Ecology & Epidemiology·Mahmoud M NaguibJosef D Järhult
Oct 26, 2021·Genome Biology and Evolution·Helen PiontkivskaMarta L Wayne

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