Different cytoplasmic/nuclear distribution of S6 protein phosphorylated at S240/244 and S235/236

Amino Acids
Margit RosnerMarkus Hengstschläger

Abstract

Higher eukaryotic ribosome biogenesis takes place in the nucleolus and requires the import of ribosomal proteins from the cytoplasm. The ribosomal protein S6 is essential for the formation of ribosome subunits, and in mice S6 heterozygosity triggers embryonal lethality. Downstream of the mTOR (mammalian target of rapamycin) and MAPK (mitogen-activated protein kinase) signalling pathways S6 protein is phosphorylated at clustered residues S235/236 and S240/244 upon numerous physiological and pathological stimuli. Here, we show that S240/244-phosphorylated S6 is predominantly nuclear but also detectable in the cytoplasm, whereas S235/236-phosphorylated S6 is almost exclusively localized to the nucleus of primary human cells and virtually undetectable in the cytoplasm. However, in transformed cells the latter can also be detected in the cytoplasm. Experiments with the mTOR inhibitor rapamycin revealed that neither blocking the phosphorylation of S6 at S235/236 and S240/244 nor arresting the cell cycle affects the cytoplasmic/nuclear localization of S6 protein. Our findings provide new insights into the regulation of S6 phosphorylation and S6 protein localization in mammalian cells.

References

Sep 8, 2004·Amino Acids·M RosnerM Hengstschläger
Apr 11, 2006·Molecular Cell·Dos D SarbassovDavid M Sabatini
May 9, 2006·Trends in Biochemical Sciences·Igor Ruvinsky, Oded Meyuhas
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Apr 27, 2007·Amino Acids·M Rosner, M Hengstschläger
May 2, 2007·Human Molecular Genetics·Margit RosnerMarkus Hengstschläger
Aug 8, 2007·Cell Research·Qian Yang, Kun-Liang Guan
Aug 16, 2008·International Review of Cell and Molecular Biology·Oded Meyuhas
Jan 16, 2009·Amino Acids·M RosnerM Hengstschläger
Oct 9, 2009·Journal of Cell Science·Mathieu Laplante, David M Sabatini

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Citations

Jan 30, 2014·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Miaoqing HuAihua Liang
Oct 20, 2012·Amino Acids·Michael S ParkerSteven L Parker
May 9, 2012·Expert Opinion on Therapeutic Targets·Carman K M Ip, Alice S T Wong
Jul 23, 2014·Asian-Australasian Journal of Animal Sciences·Bao WenleiWang Zhigang
May 29, 2013·Cellular Signalling·Claudia WizaD Margriet Ouwens
Nov 18, 2011·Human Molecular Genetics·Christiane FuchsMarkus Hengstschläger
Mar 13, 2018·PloS One·Denisa JansovaAndrej Susor
Nov 1, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Aurélie TréfierPascale Crépieux

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