PMID: 6988445Feb 1, 1980Paper

Different cytoskeletal domains in murine fibroblasts

Journal of Cell Science
J H Temmink, H Spiele


The morphology of the cytoskeleton of normal 3T3 fibroblasts was studied in the electron microscope on embedded and sectioned cells as well as on critical-point-dried whole mounts. Four ultrastructurally different cytoplasmic domains could be distinguished within every 3T3 cell, namely: Cytocortex, ruffle area, endoplasm, and stress fibre. However, many transitions between the different domains were also found. In addition, 2 other domains were occasionally encountered in the same cells. Based on the knowledge that actin is present in relatively high concentration throughout non-muscle cells and that it can, under different physiological conditions, assume different morphological appearances, an attempt has been made to formulate a hypothesis that links the ultrastructural domains to different cellular functions. Many experimental approaches are available to test the hypothesis and at the same time increase our knowledge of the cytoskeleton of 3T3 cells.

Related Concepts

Plasma Membrane
Cytological Techniques
Microtrabecular Lattice
Electron Microscopy

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Torsion Dystonia

Torsion dystonia is a movement disorder characterized by loss of control of voluntary movements appearing as sustained muscle contractions and/or abnormal postures. Here is the latest research.

Generating Insulin-Secreting Cells

Reprogramming cells or using induced pluripotent stem cells to generate insulin-secreting cells has significant therapeutic implications for diabetics. Here is the latest research on generation of insulin-secreting cells.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Epigenome Editing

Epigenome editing is the directed modification of epigenetic marks on chromatin at specified loci. This tool has many applications in research as well as in the clinic. Find the latest research on epigenome editing here.